There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
A new HPLC coupled with evaporative light scattering detection (ELSD) method has been
developed for the simultaneous quantitative determination of seven major saponins,
namely macranthoidin B (1), macranthoidin A (2), dipsacoside B (3), hederagenin-28-O-beta-D-glucopyranosyl(6-->1)-O-beta-D-glucopyranosyl
ester (4), macranthoside B (5), macranthoside A (6), and hederagenin-3-O-alpha-L-arabinopyranosyl(2-->1)-O-alpha-L-rhamnopyranoside
(7) in Flos Lonicerae, a commonly used traditional Chinese medicine (TCM) herb. Simultaneous
separation of these seven saponins was achieved on a C18 analytical column. The mobile
phase consisted of (A) acetonitrile-acetic acid (95:0.5) and (B) 0.5% aqueous acetic
acid using a gradient elution of 29%A at 0-10 min, 29-46%A at 10-25 min and 46%A at
25-30 min. The drift tube temperature of ELSD was set at 106 degrees C, and with the
nitrogen flow-rate of 2.6 l/min. All calibration curves showed good linear regression
(r2>0.9922) within test ranges. This method showed good reproducibility for the quantification
of these seven saponins in Flos Lonicerae with intra- and inter-day variations of
less than 3.0% and 6.0%, respectively. The validated method was successfully applied
to quantify seven saponins in five sources of Flos Lonicerae, which provides a new
basis of overall assessment on quality of Flos Lonicerae.