Stimulatory Role of Substance P on Gonadotropin Release in Ovariectomized Rats

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Abstract

Substance P (SP) has been shown to be present in the hypothalamus and anterior pituitary. To evaluate a possible physiological role of endogenous SP in the control of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) release, specific antiserum against SP (anti-SP) was injected intraventricularly (3 µl into the third ventricle) or intravenously (50 or 200 µl) into conscious, ovariectomized (OVX) rats. Third ventricular injection of the antiserum induced a significant decrease in both plasma LH and FSH levels when compared to values in control animals injected with normal rabbit serum (p < 0.01 and p < 0.025, respectively). The effect was observed within 10 min and levels remained suppressed for 60 min. In contrast, intravenous injection of large doses of anti-SP had no effect on the release of both hormones. In order to confirm the stimulatory effect of SP itself, synthetic SP was injected intravenously and intraventricularly into estrogen-primed (E-primed), OVX rats. Synthetic SP dramatically stimulated LH release, but not FSH release when injected either intravenously or intraventricularly at doses of 10 and 50 µg (p < 0.001, p < 0.005 vs. control, respectively). To investigate any direct action of SP on gonadotropin release from the anterior pituitary gland, synthetic SP was incubated with dispersed anterior piutitary cells harvested from E-primed OVX rats. SP did not affect the release of gonadotropins in vitro. These results indicate that endogenous hypothalamic SP exerts a tonic stimulatory hypothalamic control of basal gonadotropin release in OVX rats.

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Journal

Journal ID (publisher-id): NEN

Journal ID (nlm-ta): Neuroendocrinology

Journal ID (doi): 10.1159/issn.0028-3835

Title: Neuroendocrinology

Publisher: S. Karger AG

ISSN (Print): 0028-3835

ISSN (Electronic): 1423-0194

Publication date Subscription-year: 1990

Publication date (Print): 1990

Publication date (Electronic): 03 April 2008

Volume: 51

Issue: 5

Pages: 523-529

Affiliations

^aDepartment of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan; ^bDow Chemical Company, ME. Pruitt Research Center, Midland, Mich.; ^cDepartment of Anatomy, Ohio State University, College of Medicine, Columbus, Ohio; ^dDepartment of Obstetrics and Gynecology, University of Iowa, College of Medicine, Iowa City, Iowa; ^eDepartment of Physiology, The University of Texas Southwestern Medical Center, Dallas, Tex.; ^fDepartment of Physiology and Biophysics, University of Alabama, Birmingham, Ala., USA

Article

Publisher ID: 125386 Other ID: Neuroendocrinology 1990;51:523–529

DOI: 10.1159/000125386

PubMed ID: 1693756

SO-VID: 8035df8e-13b3-4f7d-992f-b37fd4fd2617

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 09 March 1989

Date accepted : 22 September 1989

Page count

Pages: 7

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