A Chemiluminescent Platform for Smartphone Monitoring of H ₂O ₂ in Human Exhaled Breath Condensates

Abstract

Noninvasive measurement of oxidative markers in clinical samples has the potential to rapidly provide information for disease management, but is limited by the need for expensive analytical instrumentation that precludes home monitoring or point-of-care applications. We have developed a simple to use diagnostic platform for airway hydrogen peroxide (H ₂O ₂) that combines optimized reaction-based chemiluminescent designs with an inexpensive home-built darkbox and readily available smartphone cameras. Specialized photography software applications and analysis of pixel intensity enables quantification of sample concentrations. Using this platform, sample H ₂O ₂ concentrations as low as 264 nM can be detected. The platform has been used to measure H ₂O ₂ in the exhaled breath condensates of human subjects, showing good agreement with the standard Amplex Red assay.

Graphical Abstract