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      Concentraciones e intervalos de aplicación del aceite esencial de Tagetes lucida Cav. contra Nacobbus aberrans Translated title: Concentrations and application intervals of the essential oil of Tagetes lucida Cav. against Nacobbus aberrans

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          Abstract

          Resumen La escaza información sobre los efectos del aceite esencial de Tagetes lucida contra nematodos y la riqueza de poblaciones nativas de esta especie vegetal en algunas áreas en el Estado de México resultan condiciones favorables para derivar insumos naturales inocuos de esta especie que posibiliten enfrentar problemas de agallamiento por N. aberrans en la producción de jitomate. El objetivo de este estudio fue evaluar en condiciones de invernadero la aplicación de aceite de T. lucida de una población natural de Ixtapan de la Sal, Estado de México en la formación de agallas por N. aberrans en plántulas de jitomate. El aceite esencial se extrajo por hidrodestilación a nivel piloto con rendimiento en peso seco de 0.4% (mL 100 g-1) y se analizó por CG/EM identificando los compuestos mayoritarios siguientes: acetato de geranilo (40.8%), β-ocimeno (15.1%), nerolidol (8.1%), β-cubebeno (5.1%) y cariofileno (5.2%). Plántulas de jitomate en maceta se inocularon con N. aberrans (10 mL kg-1 de sustrato) y se les dosificaron concentraciones de aceite desde 0.01 hasta 10 mg mL-1, como tratamientos preventivos y de control, en intervalos de aplicación de aceite de 1, 2 y 3 semanas. La inhibición del agallamiento de la raíz fue consistente en el tratamiento de control (TC) que en el preventivo. En TC, concentraciones de aceite de 0.35 y 1 mg mL-1 produjeron 63 a 80% de inhibición del agallamiento y se obtuvieron valores de CL50 de 0.06 mg mL-1 para los intervalos 1 y 2, y de 0.13 mg mL-1 para el intervalo 3.

          Translated abstract

          Abstract The scarce information on the effects of the essential oil of T. lucida against nematodes and the richness of native plant populations of this species in some localities at State of Mexico are favorable conditions to derive innocuous natural inputs that make it possible to face problems of galling by N. aberrans in the production of tomato. The objective of this study was to evaluate in greenhouse conditions the application of T. lucida oil from a natural population of Ixtapan of the Sal, State of Mexico in the formation of galls by N. aberrans in tomato seedlings. The essential oil was extracted by hydrodistillation at pilot level with dry weight yield of 0.4% (mL 100 g-1) and analyzed by GC/EM identifying the following major compounds: geranyl acetate (40.8%), β-ocimene (15.1%), nerolidol (8.1%), β-cubebeno (5.1%) and caryophyllene (5.2%). Tomato seedlings in pot were inoculated with N. aberrans (10 mL kg-1 of substrate) and were dosed with oil concentrations from 0.01 to 10 mg mL-1, as preventive and control treatments, in oil application intervals. 1, 2 and 3 weeks. Inhibition of root galling was consistent in the control (TC) treatment than in the preventive one. On TC, oil concentrations of 0.35 and 1 mg mL-1 produced 63 to 80% galling inhibition, and CL50 values of 0.06 mg mL-1 were obtained for intervals 1 and 2, and 0.13 mg mL-1 for the interval 3.

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          Biological properties of essential oils: an updated review

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            Metabolic engineering of plant monoterpenes, sesquiterpenes and diterpenes--current status and future opportunities.

            Terpenoids (a.k.a. isoprenoids) represent the most diverse class of natural products found in plants, with tens of thousands of reported structures. Plant-derived terpenoids have a multitude of pharmaceutical and industrial applications, but the natural resources for their extraction are often limited and, in many cases, synthetic routes are not commercially viable. Some of the most valuable terpenoids are not accumulated in model plants or crops, and genetic resources for breeding of terpenoid natural product traits are thus poorly developed. At present, metabolic engineering, either in the native producer or a heterologous host, is the only realistic alternative to improve yield and accessibility. In this review article, we will evaluate the state of the art of modulating the biosynthetic pathways for the production of mono-, sesqui- and diterpenes in plants. © 2012 The Authors Plant Biotechnology Journal © 2012 Society for Experimental Biology, Association of Applied Biologists and Blackwell Publishing Ltd.
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              Antifungal and antibacterial activities of Mexican tarragon (Tagetes lucida).

              Mexican tarragon (Tagetes lucida Cv. Asteraceae: Campanulatae) is an important, nutritious plant and an effective herbal medicine. Seven coumarins, 7,8-dihydroxycoumarin (4), umbelliferone (7-hydroxycoumarin) (5), scoparone (6,7-dimethoxycoumarin) (7), esculetin (6,7-dihydroxycoumarin) (11), 6-hydroxy-7-methoxycoumarin (12), herniarin (7-methoxycoumarin) (13), and scopoletin (6-methoxy-7-hydroxycoumarin) (14), and three flavonoids, patuletin (18), quercetin (19), and quercetagetin (20), were isolated from CH2Cl2 and MeOH extracts from aerial parts of T. lucida. In addition, 6,7-diacetoxy coumarin (15), 6-methoxy-7-acetylcoumarin (16), and 6-acetoxy-7-methoxycoumarin (17) derivatives were synthesized. 8-Methoxypsoralen (1), 8-acetyl-7-hydroxycoumarin (2), 7,8-dihydroxy-6-meth-oxycoumarin (3), 6,7-dimethoxy-4-methylcoumarin (6), 5,7-dihydroxy-4-methylcoumarin (8), 4-hydroxycoumarin (9), 4-hydroxy-6,7-dimethylcoumarin (10), naringenin (21), glycoside-7-rhamnonaringin (22), and rutin (23) were commercially obtained (Sigma-Aldrich). All of these compounds and extracts (M1 and M2) were assayed against bacteria and fungi. The antibacterial activity was determined on Bacillus subtilis, Escherichia coli, Proteus mirabilis, Klebsiella pneumoniae, Salmonella typhi, Salmonella sp., Shigella boydii, Shigella sp., Enterobacter aerogenes, Enterobacter agglomerans, Sarcina lutea, Staphylococcus epidermidis, Staphylococcus aureus, Yersinia enterolitica, Vibrio cholerae (three El Tor strains, CDC-V12, clinic case, and INDRE-206, were obtained from contaminated water), and V. cholerae (NO-O1). The evaluated fungi were Aspergillus niger, Penicillium notatum, Fusarium moniliforme, Fusarium sporotrichum, Rhizoctonia solani, and Trichophyton mentagrophytes. The most active compounds against Gram-positive and -negative bacteria were the dihydroxylated coumarins 3 and 4. In addition, 2-4, 6, 7, and 11 showed an interesting activity against V. cholerae, a key bacterium in the contaminated water; 2-4 were the most active. Coumarins were the most effective compounds against Gram-negative bacteria. The extract MeOH/CH2Cl2 (1: 4) M2 at 0.4 microg/disk inhibited the growth of E. coli and P. mirabilis (40%), K. pneumoniae (31.1%), Salmonella sp. (35.5%), and Shigella sp. (0%) at 72 h of culture. The dimethoxy compounds 6 and 7 showed a strong activity against fungal strains, especially T. mentagrophytes and R. solani (100% of inhibition at 125.0 and 250.0 microg/mL, respectively).
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                remexca
                Revista mexicana de ciencias agrícolas
                Rev. Mex. Cienc. Agríc
                Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (Texcoco, Estado de México, Mexico )
                2007-0934
                May 2018
                : 9
                : 3
                : 589-600
                Affiliations
                [3] Oaxaca orgnameCentro de Bachillerato Tecnológico Agropecuario Mexico mendoza.edgar@ 123456colpos.mx
                [1] orgnameUniversidad Autónoma Chapingo Mexico johan-quiahuitl@ 123456hotmail.com
                [2] Estado de México orgnameFundación Salvador Sánchez Colín CICTAMEX SC Mexico cgelidia@ 123456hotmail.com
                Article
                S2007-09342018000300589
                10.29312/remexca.v9i3.1218
                845b10c3-ad24-4a5b-8876-20f68cc8a555

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 01 January 2018
                : 01 March 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 49, Pages: 12
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                SciELO Mexico


                concentración de aceite,Nacobbus aberrans,Tagetes lucida Cav.,oil concentration,gill inhibition,inhibición de agallamiento

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