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      Wheat Fhb1 encodes a chimeric lectin with agglutinin domains and a pore-forming toxin-like domain conferring resistance to Fusarium head blight

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          Scab of Wheat and Barley: A Re-emerging Disease of Devastating Impact

          Marcia McMullen, Roger Jones, Dale Gallenberg (1997)
          Article 0 comments Cited 277 times – based on 0 reviews     Review now
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            Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants.

            Alan Christensen, Peter H. Quail (1996)
            A set of plasmids has been constructed utilizing the promoter, 5' untranslated exon, and first intron of the maize ubiquitin (Ubi-1) gene to drive expression of protein coding sequences of choice. Plasmids containing chimaeric genes for ubiquitin-luciferase (Ubi-Luc), ubiquitin-beta-glucuronidase (Ubi-GUS), and ubiquitin-phosphinothricin acetyl transferase (Ubi-bar) have been generated, as well as a construct containing chimaeric genes for both Ubi-GUS and Ubi-bar in a single plasmid. Another construct was generated to allow cloning of protein coding sequences of choice on Bam HI and Bam HI-compatible restriction fragments downstream of the Ubi-1 gene fragment. Because the Ubi-1 promoter has been shown to be highly active in monocots, these constructs may be useful for generating high-level gene expression of selectable markers to facilitate efficient transformation of monocots, to drive expression of reference reporter genes in studies of gene expression, and to provide expression of biotechnologically important protein products in transgenic plants.
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              Simple RNAi vectors for stable and transient suppression of gene function in rice.

              Ko Shimamoto, Daisuke Miki (2004)
              Since the recent sequencing of the rice genome, the functional identification of rice genes has become increasingly important. Various tagged lines have been generated; however, the number of tagged genes available is not sufficient for extensive study of gene function. To help identify the functions of genes in rice, we developed a Gateway vector, pANDA, for RNA interference of rice genes. This vector can be used for Agrobacterium transformation of rice and allows easy and fast construction of efficient RNAi vectors. In the construct, hairpin RNA derived from a given gene is transcribed from a strong maize ubiquitin promoter, and an intron is placed 5' upstream of inverted repeats to enhance RNA expression. Analysis of rice genes using this vector showed that suppression of mRNA expression was observed in more than 90% of transgenic plants examined, and short interfering RNA indicative of RNA silencing was detected in each silenced plant. A similar vector, pANDA-mini, was also developed for direct transfer into leaf cells or protoplasts. This vector can be used for transient suppression of gene function in rice. These vectors should help identify the functions of rice genes whose tagged mutants are not available at present and complement existing methods for functional genomics of rice.
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                Author and article information

                Journal
                Title: Nature Genetics
                Abbreviated Title: Nat Genet
                Publisher: Springer Nature
                ISSN (Print): 1061-4036
                ISSN (Electronic): 1546-1718
                Publication date Created: October 24 2016
                Publication date (Print): October 24 2016
                Volume: 48
                Issue: 12
                Pages: 1576-1580
                Article
                DOI: 10.1038/ng.3706
                PubMed ID: 27776114
                SO-VID: 88692cf9-c370-4a02-99e4-485a297b9885
                Copyright © © 2016
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