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      Anatomical investigations on root, stem, and leaf of Gentiana olivieri Griseb

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          Abstract

          Background:

          Gentiana olivieri Griseb. (Afat) (Gentianaceae), which has many bioactive compounds is used as antidiabetic, hepatoprotective, digestive aid, antidepressant, and antianemic in traditional medicine.

          Materials and Methods:

          Root, stem, and leaf sections of G. olivieri were taken free hand or by sliding microtome and examined on light microscope.

          Results:

          Anatomical characters of the species were observed to be similar to the usual features of Gentianaceae anatomy.

          Conclusion:

          Intraxylary phloem, which was primarily the distinguishing feature between Gentianoideae and Menyanthoideae sub-families was observed in G. olivieri roots.

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          Most cited references 44

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          Plant microtechnique

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            Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques.

            Hypoglycemic effect of Gentiana olivieri Griseb. (Gentianaceae) flowering herbs on oral administration were studied using in vivo models in normal, glucose-hyperglycemic and streptozotocin-induced diabetic rats. Through in vivo bioassay-guided fractionation processes isoorientin, a known C-glycosylflavone, was isolated from the ethylacetate fraction by silica gel column chromatography as the main active ingredient from the plant. Isoorientin exhibited significant hypoglycemic and antihyperlipidemic effects at 15 mg/kg b.w.dose. Isoorientin concentration of the extracts and fractions were determined by HPLC in order to establish a correlation between the hypoglycaemic activity.
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              Elements of botanical microtechnique

               JE SASS,  JE Sass,  J. Sass (1940)
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                Author and article information

                Journal
                Pharmacogn Mag
                PM
                Pharmacognosy Magazine
                Medknow Publications (India )
                0973-1296
                0976-4062
                Jan-Mar 2011
                : 7
                : 25
                : 9-13
                Affiliations
                Ankara University, Science Faculty, Department of Biology, Tandogan, 06100, Ankara, Turkey
                [1 ] Gazi University, Faculty of Pharmacy, Department of Pharmacognosy, 06330 Etiler, Ankara, Turkey
                Author notes
                Address for correspondence: Canan Yağci Tüzün, Ankara University, Science Faculty, Department of Biology, Tandogan, 06100, Ankara, Turkey. E-mail: cyagci@ 123456science.ankara.edu.tr
                Article
                PM-7-9
                10.4103/0973-1296.75877
                3065163
                21472072
                © Pharmacognosy Magazine

                This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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