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Abstract
To encourage stem cell differentiation, gold nanoparticles (20 nm) were used to deliver
electrical stimulation to human embryonic stem cells (hESCs) in vitro. Nano-structured
gold nanoparticles were designed by coating the surface of culture dishes with gold
nanoparticles using a layer-by-layer (LBL) system. In this method, gold nanoparticles
were continuously coated onto dishes by SEM analysis. Evaluation of gene modified
hESCs that were subsequently attached onto fibronectin-coated gold nanoparticles revealed
that the un-differentiation marker, Oct-4, was no longer present following electrical
stimulation. In addition, the osteogenic markers of collagen type I and Cbfa1 increased
in response to electrical stimulation, while those of hESCs were not observed without
electrical stimulation.