Critical analysis of the published literature about the effects of Ramadan intermittent fasting on healthy children's physical capacities

Abstract

Ramadan is the month in which Muslims refrain from food and fluid intake from dawn to sunset. It is quite questionable whether pre-pubescent children should be allowed to fast while religion advises such a practice only after puberty, but it is a relatively current practice that children make their first attempt to fast the entire month while they are still pre-pubescent. Most published studies examining the effects of Ramadan intermittent fasting (RIF) on sport performance were performed on adult subjects, and data regarding its effects on children are few. We searched MEDLINE, EMBASE, and ISI Web of Science on April 10, 2015, using the combination of the following medical subject headings: (‘fasting’ AND ‘exercise test’) AND (‘child’ OR ‘adolescent’)]. Only four studies (1–4) examined the effect of RIF on children's physical performance. They were published between 2008 (2) and 2014 (1) in North Africa (n=2) (1, 4) and the Middle East (n=2) (2, 3). The four studies’ methodologies and main results are detailed, respectively, in Tables 1 and 2. The effects of RIF on the physical capacities of children seem controversial, but there is a tendency toward a decrease in endurance performance, while a slight decrement or no significant effect on short-term explosive performance has been shown (1–4). Table 1 Study designs and characteristics of included subjects in published studies aiming to evaluate the effects of RIF on physical performance of male children Region/first author(s) Middle EastMeckel et al. (2) Middle East Girard and Farooq (3) North AfricaAloui et al. (4) North AfricaFenneni et al. (1) Ramadan year NR 2010 NR 2012 Timing NR 13:00–14:00 h Morning (07:00–09:00 h) and afternoon (17:00–19:00 h) 15:00–17:00 h Elapsed fasting time NR NR Dawn to sunset ~15 h Dawn to sunset ~16 h at the beginning (20th of July) and ~15 h at the end (18th of August) Average ambient temperature and humidity NR NR NR ~25°C; 38–42% Number of evaluation sessions Two sessions (same time of day conducted on 2 successive days):BRLast 2 days of Ramadan Five sessions (same time of day):1 week BRR1R4AR2AR4 Two testing phases:1 week BRR42 test sessions in each period (recovery period of at least 36 h in between):1 in the morning1 in the afternoon Four testing phases:2 weeks BRR2R410–12 days AR2 test sessions in each period, with a recovery period of at least 36 h in between Number of subjects 19 18 12 18 Age (years) 15.1±0.9a 14–16b 12.6±1.5a 13.3±0.4a 11.9±0.8a [10.2–13.4]b (11.5–12.3)c Height (cm) 166±4a 156±13a 165±3a 153±9a [149–157]b (136–168)c Body mass (kg) 62.5±7.4a 45.3±12.4a 60.9±6.5a 55±18a [46–64]b (34–91)c Training status Soccer players with regular training program during Ramadan Untrained Soccer players (minimum of 3 years of practice) observing Ramadan fasting for the first time Sedentary (practice of sport activity only at school) observing Ramadan fasting for the first time AR, after-the-end of-Ramadan; AR2, 2 weeks AR; AR4, 4 weeks AR; BR, before-Ramadan; h, hour; NR, not reported; RIF, Ramadan intermittent fasting; R1, 1st week of Ramadan; R2, end of the second week of Ramadan; R4, 4th week of Ramadan. a Data are range (minimum to maximum) b Data are range (minimum to maximum) c Data are 95% confidence interval. Table 2 Tests and main results of main published studies aiming to evaluate the effects of RIF on physical performance of male children Regionfirst author(s) Middle EastMeckel et al. (2) Middle EastGirard and Farooq (3) North AfricaAloui et al. (4) North AfricaFenneni et al. (1) Collected data VJT height40-m sprint time4×10-m run timesum 6×40-m run time6×40-m performance decrement (%)3,000-m run timeBody massSkinfold measurement, caloric intake (kcal/day)% of carbohydratesFats and proteins Intense physical activity (h/week)Sleep habits (week BR, last week of Ramadan) (h/day)Total sleeping hoursEnergy intake in a regular month and during Ramadan Best time in a single sprintCumulated sprint timesSprint decrement scoreBody composition (body fat%, lean mass, fat mass)Objective daily activityEstimated energy expenditureCaloric intake% of proteinsFat and carbohydrates Squat jumpCMJ heightsEstimated V . O2maxPerceived exertionFood intakes over a span of 3 days for each week of physical testing 6MWDVJT heightHJT distance20-m sprint time30-m sprint timeMBT distance Test instructions Standard warm-up procedure15–20 min period separated the different tests on each dayEach run started from a standing position Complete all sprints as fast as possible Players familiarized with the VJT and the multistage 20-m shuttle run testActive warm-up after 30-min rest in a seated position Subjects familiarized with all the study tests Test encouragement NR Strong verbal encouragement during all sprints NR Verbally encouragement during short-term exercises and during the 6MWT (5) Physical performance results Non-significant change in body massSignificant increase in the sum of skinfoldsReduced aerobic endurance (increased 3,000-m running time)Reduced speed endurance (increased sum of 6×40-m run time and performance decrement)Reduced CMJ performanceNon-significant effect on the 40-m sprint time or agility performanceReduced intense physical activityNon-significant changes in sleeping hours or total caloric intake (including the relative consumption of carbohydrates, fats and proteins) Compared to BR, cumulated sprint times lengthened during Ramadan (R1; R4) and remained elevated AR (AR2 and AR4)Initial best sprint performance and sprint decrement score did not changeBody mass (but not body fat, lean mass and fat mass) was elevated at R4 and AR2 compared with BR, whereas energy expenditure remained constant Jumping heights during the squat jump and the CMJ tests and estimated V . O2max were lower during Ramadan than BR in the afternoon, and their diurnal variations observed BR were not apparent during the fasting period 6MWD (mean or % predicted) was lower during R2 and R4 compared with BR.6MWD (% predicted) was significantly lower during R2 and during R4 compared with ARBody mass was lower during R2 and R4 compared with ARNon-significant effect on VJT height, HJT distance, 20- and 30-m sprint time and MBT distance Sleep loss No significant change in sleeping hours BR and during Ramadan NR NR NR Caloric intake No significant change in total caloric intake, or in the relative consumption of carbohydrates, fat, and protein BR and during RamadanNon-significant increase in fat consumptionDecrease in carbohydrate and protein intake during Ramadan NR No significant change in the daily total caloric, or in the relative consumption of carbohydrates, fat, and protein before and during Ramadan NR Conclusions RIF reduces physical work capacity of adolescent soccer playersThe timing of meals during the day and the relative contribution of macro-nutrients (e.g. carbohydrates) rather than the total caloric intake, serve as the main nutrient causes for the decrease in physical capacityDecreased physical activity and disturbed sleeping patterns may also contribute to the significant decrease in athletic performance Mean sprint performance during repeated sprinting is compromised toward the end of RamadanThis effect persisted AR2Fatigue resistance was not affected Diurnal variations of short-term maximal performances and endurance performance were affected by RIFDehydration, disturbances of the sleep–wake cycle by changed food and fluid intakes and/or alterations in circadian rhythms, and fatigue due to sleep loss may explain performance impairment observed during Ramadan in adolescent soccer players RIF showed no significant effect upon short-term explosive efforts but reduced endurance efforts’ performance and body mass CMJ, counter movement jump; HJT, horizontal jump test; MBT, medicine ball throw; NR, not reported; V . O2max, maximal oxygen uptake; VJT, vertical jump test; 6MWD, 6-min walk distance. For other abbreviations, see Table 1. The sample sizes of children in these four studies varied between 12 (4) and 19 (2). Two remarks concerning this issue should be raised: Only one study (1) calculated the required sample size according to a predictive equation (6). This could be a statistically crucial point since determining the optimal sample size for a study assures an adequate power to detect statistical significance and is a critical step in the design of a research protocol (6). Using too many participants in a study is expensive and exposes more subjects to procedures (6). On the other hand, if a study is underpowered, it will be statistically inconclusive and may make the whole protocol a failure (6). In the latter case, the study cannot be used to draw valid conclusions despite having exposed a number of participants to the study procedures. In the future, similar studies should include appropriate sample sizes calculated according to a predictive equation (6). No study included a parallel control group of non-fasting individuals. This could be considered a serious omission because the internal validity of the findings from these studies and the changes in the variables assessed cannot be attributed solely to RIF. Nevertheless, it has to be noted that obtaining non-fasting groups in ‘Muslim’ countries is not easy due to understandable ethical reasons (1). Therefore, most of the studies on RIF used before-Ramadan (BR) values as baseline or control. For example, a MEDLINE search performed on April 10, 2015, using the key words ‘Ramadan fasting’ and ‘control group’ identified only 13 studies (7–18). All were performed on adults, and only six studies (7–12) were about exercise physiology. In the future, similar studies should systematically include a non-fasting control group whenever possible to reduce the possibility of learning effects skewing the results and to avoid any threat to the internal validity of the findings (1). Other important methodological limitations leading to anecdotal comparisons of physical responses between studies were also noted: Information about the geographical location and/or the season was lacking in two studies (2, 4). Ramadan lasts from 29 to 30 days based on the lunar crescent visibility. In addition, Ramadan moves ahead ~11 days each year compared to the Gregorian calendar and can occur in any season (19). Consequently, the effects of daytime fasting are powerfully influenced by climatic circumstances: Ramadan in summer at elevated latitudes presents very different features compared to Ramadan in winter at lower latitudes (19). The elapsed time between dawn and sunset was not mentioned in two studies (2, 3). This parameter is of importance, as it depends on the geographical site and the season of the year. Indeed, the fasting duration can be as long as 18 h a day in the summer in temperate locations (19), and is even longer in countries situated nearer the poles, which poses a real challenge for fasting individuals (19). For example, in the study by Fenneni et al. (1) performed in a North African country during the summer of 2012, the elapsed time from dawn to sunset was ~16 h at the beginning and ~15 h at the end of Ramadan. This condition could be considered as relatively challenging as fasting duration was relatively long for the young study participants (1). Test timing (time of day) was not mentioned in one study (2). It is well known that test timing could affect physical performance (1). For example, subjects in the Fenneni et al. (1) study performed the tests only about 2 h before breaking their fast. In addition, it has been clearly established that RIF led to impairment in adolescent soccer players’ performance in the afternoon and consequently impacted their diurnal pattern observed BR (4). Information about previous experience with RIF was omitted in two studies (2, 3). Some authors have shown that the number of years the subjects had fasted (the RIF history of the participants) could influence their adaptations and responses to exercise testing (20). Direct communication with Girard and Farooq (3) showed that the subjects in their study all had fasted at least one Ramadan month before the study was conducted (1). In two studies (1, 4), the subjects were fasting for the first time, and this particular condition may present a challenge for them (1, 4). Information about the children's physical activity status was omitted in one study (3). In two others (2, 4), the participants’ physical activity status was qualified as ‘athletes’ [soccer players with a minimum of 3 years of previous practice (4) or with regular training program during Ramadan (2)]. In one study (1), the boys were ‘healthy untrained’ (they never participated in any sporting activity elsewhere than at school where they habitually practice for a maximum of 2 h/week). The physical activity status of the participants should be recognized, as it may interfere with the independent effects of RIF on physical capacity (1, 2). Two studies lacked acute after-Ramadan (AR) control data (2, 4). In this case, it is still unknown whether the sleep cycle alterations and/or diurnal nutrition imposed by Ramadan led to momentary performance adaptations and/or persistent effects a few weeks AR (21). The average ambient temperature and relative humidity at the time of physical testing were lacking in three studies (2–4). This is a serious methodological limitation since high climatic heat stress may affect children's performance (22). In the Fenneni et al.'s (1) study, the medium testing temperature was ~25°C and the humidity ranged from 38 to 42%. These circumstances could be considered somewhat challenging, as temperature was relatively warm (1). The mean ages of the subjects ranged from 11.9±0.8 years (1) to 15.1±0.9 years (2) and from 10.2 years (1) to 16.0 years (2). Height varied between studies by up to 12 cm (153±9 cm1 to 166±4 cm2) and body mass varied by up to 17 kg (45.3±12.4 kg3 to 62.5±7.4 kg2). This makes comparisons between studies difficult. In addition, as several physical capacities depend on anthropometric values, they should be expressed as percentage of predicted reference values (such as for the 6-min walk distance, 6MWD) (5). The number of testing periods varied from two (2, 4) to five (3). In addition, testing periods were not defined in the same way in all studies. ‘BR’ was defined as one (2–4) or two (1) weeks BR. ‘During Ramadan’ was defined as the first week of Ramadan (R1) (3), the end of the second week of Ramadan (R2) (1), or the last 2 days of Ramadan (2) as the end of the fourth week of Ramadan (R4) (1, 3) (4). ‘AR’ was defined as 10–12 days AR (AR2) (1), 2 weeks AR (AR2) (1), or 4 weeks AR (AR4) (3). The nature of the repeated sprint test (e.g. sprint number/duration, recovery time) is known to affect the physical performance (i.e. task dependency of the Ramadan effects) and, therefore, leads to anecdotal comparisons of physical responses between studies (3). In addition, data about encouragement during tests were lacking in two studies (2, 3). This is a very important point because encouragement can modify the outcome, e.g. of the 6MWD (5). Another limitation is that body temperature was not measured in some studies (23). Using inappropriate terms [such as ‘anaerobic’ exercise (24)] can be a source of confusion. For example, Meckel et al. (2) used the term ‘anaerobic capacity’ to refer to speed endurance (sum 6×40 m run time). In conclusion, future studies should be made more rigorous by taking into account the various factors discussed here. They should also focus on the effects of RIF on young international-caliber athletes, young female athletes, cognitive function, circadian rhythms (body temperature, metabolism, hormones, etc.), and changes in physiological functions (spirometry, heart rate, oxy-hemoglobin saturation, blood lactate concentration, and urinary excretion). Mohamed Amine Fenneni* Research Unit ‘Exercise Physiology and Pathophysiology – from the Integrated to the Molecular Biology, Medicine and Health’UR12ES06Faculty of Medicine of SousseUniversity of SousseSousse, TunisiaLaboratory of PhysiologyFaculty of Medicine of SousseUniversity of SousseSousse, Tunisia Imed Latiri* Research Unit ‘Exercise Physiology and Pathophysiology – from the Integrated to the Molecular Biology,Medicine and Health’UR12ES06Faculty of Medicine of SousseUniversity of SousseSousse, TunisiaLaboratory of PhysiologyFaculty of Medicine of SousseUniversity of SousseSousse, Tunisia Asma Aloui* Research Laboratory ‘Sport Performance Optimization’National Center of Medicine and Sciences in SportTunis, Tunisia Sonia Rouatbi Research Unit ‘Exercise Physiology and Pathophysiology – from the Integrated to the Molecular Biology,Medicine and Health’UR12ES06Faculty of Medicine of SousseUniversity of SousseSousse, TunisiaLaboratory of PhysiologyFaculty of Medicine of SousseUniversity of SousseSousse, TunisiaDepartment of Physiology and Functional ExplorationFarhat HACHED University Hospital of SousseSousse, Tunisia Karim Chamari** Athlete Health and Performance Research CenterASPETARQatar Orthopedic and Sports Medicine HospitalDoha, Qatar Helmi Ben Saad** Laboratory of PhysiologyFaculty of Medicine of SousseUniversity of SousseSousse, Tunisia Department of Physiology and Functional ExplorationFarhat HACHED University Hospital of SousseSousse, TunisiaResearch Laboratory No. LR14ES05: Interactions of the Cardiopulmonary SystemFaculty of Medicine of SousseUniversity of SousseSousse, TunisiaEmail: [e-mail]