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      Detección de variabilidad genética en aislamientos de Cercospora kikuchii contaminantes de un mismo sembradío de soja Translated title: Detection of genetic variability in Cercospora kikuchii isolates from a single soybean field

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          Abstract

          El conocimiento de la epidemiología y la estructura poblacional de Cercospora kikuchii está poco desarrollado y no se han comunicado estudios al respecto en la Argentina. El objetivo de este trabajo fue seleccionar oligonucleótidos que permitan detectar variabilidad genética en aislamientos de C. kikuchii obtenidos a partir de soja proveniente de un mismo sembradío, mediante la aplicación de RAPD. Se trabajó con 6 aislamientos de C. kikuchii, 5 de ellos se obtuvieron a partir de trozos de tejido enfermo y el restante provenía de una colección de cultivos. De los 7 oligonucleótidos empleados, 5 resultaron útiles para el estudio poblacional de los aislamientos de C. kikuchii.

          Translated abstract

          Current knowledge about epidemiology and population structure of Cercospora kikuchii is little developed and no studies regarding this subject have been reported in Argentina. The aim of this work was to select primers to study genetic variability in C. kikuchii isolated from the same soybean field using RAPD (Random Amplified Polymorphism DNA). RAPD was applied to the DNA of 5 C. kikuchii, isolated from diseased tissue of the soybean in the same field, another isolate, from a strain collection. Out of seven primers, five of them proved to be useful to study the population of C. kikuchii isolates.

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          Molecular cloning: a laboratory manual

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            Factors affecting reproducibility of random amplified polymorphic DNA fingerprinting.

            The reproducibility of random amplified polymorphic DNA (RAPD) was tested using two different thermal cyclers and three brands of Taq DNA polymerase. Three different oligonucleotides were used to obtain patterns of amplified fragments from three DNA samples (Escherichia coli, Bacillus subtilis, and Thermococcus littoralis). Experiments were repeated three to six times. Apart from the expected between-oligonucleotide and between-DNA variations, between-thermal cycler and between-DNA polymerase variations were observed. Within the DNA-oligonucleotide-DNA-polymerase-thermal cycler, reproducibility was excellent when the thermal cycler equipped with the best temperature regulation was used, but was not as good with another brand of thermal cycler.
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              Cercosporin, a Photosensitizing Toxin fromCercosporaspecies

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                Author and article information

                Journal
                ram
                Revista argentina de microbiología
                Rev. argent. microbiol.
                Asociación Argentina de Microbiología (Ciudad Autónoma de Buenos Aires, , Argentina )
                0325-7541
                1851-7617
                March 2007
                : 39
                : 1
                : 11-14
                Affiliations
                [01] Paraje El Pozo Santa Fe orgnameUniversidad Nacional del Litoral orgdiv1Facultad de Bioquímica y Ciencias Biológicas orgdiv2Cátedra de Microbiología General Argentina
                Article
                S0325-75412007000100003 S0325-7541(07)03900100003
                8ba93387-df26-4cd7-b0e7-f37287adc6f6

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 11 December 2006
                : 17 October 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 14, Pages: 4
                Product

                SciELO Argentina

                Categories
                Microbiología Básica

                Cercospora kikuchii,variabilidad genética,RAPD,genetic variability

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