The characterization of the Brillouin-loss spectrum of single-mode fibers with very short (<10-ns) pulses has been studied. It was found that the Brillouin-loss signal intensity is linearly related to the duration of the pump pulse used to obtain the spectrum. In contrast with the uniform trend of the signal, three distinct behaviors were observed in the spectral linewidth. At long pulse durations the linewidth was constant at approximately 40 MHz. Pulse durations of the order of the phonon lifetime resulted in a broader spectrum, reaching a maximum width of ~100 MHz at 5 ns. Reducing the pulse duration further resulted in a sudden narrowing of the Brillouin line.