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      Structural characterization and bioactivity evaluation of water-extractable polysaccharides from chickpeas ( Cicer arietinum L.) seeds

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          Abstract

          Two water-extractable polysaccharide fractions designated as CWP (7. 37 × 10 5 Da) and CWP-0.2 (1.58 × 10 4 Da) were isolated and purified from chickpea ( Cicer arietinum L.) seeds. The chemical structure of the two polysaccharides was characterized by various methods. Monosaccharide composition and methylation analysis showed that CWP was composed of Man and Glc in a molar ratio of 44.6:55.4, and CWP-0.2 was composed of Rha, Ara, Man, Glc, and Gal in a molar ratio of 10.6:23.3:5.2:4.9:56. Further structural characterization indicated that the main chain connection of CWP was → (2-β-d-Fru f-1) n →, and the main chain connection of CWP-0.2 was explored as → 2,4)-α-l-Rha p-(1 → 3)-α-d-Gal p-(1 → with the branched chain of → 2,4)-α-l-Rha p-(1 → o-4. Besides, both CWP and CWP-0.2 had antioxidant and immunoregulatory activity in vitro, through scavenging DPPH· and ABTS· + as well as stimulating production of NO, IL-6, TNF-α and MCP-1 in RAW 264.7 macrophages. CWP-0.2 revealed significantly higher bioactivity than CWP.

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          Most cited references31

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          The hydrophilic and lipophilic contribution to total antioxidant activity

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            Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review.

            Chickpea (Cicer arietinum L.) is an important pulse crop grown and consumed all over the world, especially in the Afro-Asian countries. It is a good source of carbohydrates and protein, and protein quality is considered to be better than other pulses. Chickpea has significant amounts of all the essential amino acids except sulphur-containing amino acids, which can be complemented by adding cereals to the daily diet. Starch is the major storage carbohydrate followed by dietary fibre, oligosaccharides and simple sugars such as glucose and sucrose. Although lipids are present in low amounts, chickpea is rich in nutritionally important unsaturated fatty acids such as linoleic and oleic acids. β-Sitosterol, campesterol and stigmasterol are important sterols present in chickpea oil. Ca, Mg, P and, especially, K are also present in chickpea seeds. Chickpea is a good source of important vitamins such as riboflavin, niacin, thiamin, folate and the vitamin A precursor β-carotene. As with other pulses, chickpea seeds also contain anti-nutritional factors which can be reduced or eliminated by different cooking techniques. Chickpea has several potential health benefits, and, in combination with other pulses and cereals, it could have beneficial effects on some of the important human diseases such as CVD, type 2 diabetes, digestive diseases and some cancers. Overall, chickpea is an important pulse crop with a diverse array of potential nutritional and health benefits.
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              Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics

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                Author and article information

                Contributors
                Journal
                Front Nutr
                Front Nutr
                Front. Nutr.
                Frontiers in Nutrition
                Frontiers Media S.A.
                2296-861X
                28 July 2022
                2022
                : 9
                : 946736
                Affiliations
                [1] 1Institute of Crop Science, Chinese Academy of Agricultural Sciences , Beijing, China
                [2] 2Henan Key Laboratory of Cold Chain Food Quality and Safety Control, College of Food and Bioengineering, Zhengzhou University of Light Industry , Zhengzhou, China
                [3] 3School of Food Science and Technology, Henan University of Technology , Zhengzhou, China
                Author notes

                Edited by: Qiu Li, Qingdao Agricultural University, China

                Reviewed by: Yichen Hu, Chengdu University, China; Emmanuel Anyachukwu Irondi, Kwara State University, Nigeria; Ren-You Gan, Institute of Urban Agriculture (CAAS), China

                *Correspondence: Li Wu wuli02@ 123456caas.cn

                This article was submitted to Food Chemistry, a section of the journal Frontiers in Nutrition

                Article
                10.3389/fnut.2022.946736
                9366103
                8e80bf80-b5eb-4102-97cd-94496f69a11e
                Copyright © 2022 Zhu, Dun, Shi, Wang, Wu and Yao.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                History
                : 17 May 2022
                : 30 June 2022
                Page count
                Figures: 4, Tables: 4, Equations: 0, References: 31, Pages: 11, Words: 5984
                Categories
                Nutrition
                Original Research

                chickpea,polysaccharides,chemical structural,antioxidant,immunoregulatory

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