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      Caracterização microbiológica e físico-química de leite pasteurizado destinado ao programa do leite no Estado de Alagoas Translated title: Microbiological and physico-chemical characterization of pasteurized milk for the milk program in the State of Alagoas

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          Abstract

          Atualmente 17 mini usinas fazem parte do Programa do Leite no estado de Alagoas, e através de um convênio firmado entre o Laboratório de Microbiologia de Alimentos da Universidade Federal de Alagoas e a Cooperativa de Laticínio de Alagoas - CPLA para monitorar a qualidade microbiológica e físico-química de leite pasteurizado tipo C, foram analisadas 348 amostras deste produto e avaliadas quanto à contagem de coliformes a 35 e 45 °C, quanto a bactérias aeróbias mesófilas, bactérias psicrotróficas e pesquisa de Salmonella sp., segundo metodologia convencional proposta pela Instrução Normativa nº 62/2003 do MAPA. Os parâmetros físico-químicos (acidez titulável em graus Dornic, estabilidade ao álcool, densidade a 15 °C, índice crioscópico, teor de gordura, extrato seco desengordurado e teor de proteína) foram avaliados segundo metodologia convencional recomendada pelo Instituto Adolfo Lutz. As análises de gordura, proteína e extrato seco desengordurado foram realizadas utilizando o aparelho Ekomilk, conforme recomendação do fabricante. De acordo com os resultados obtidos, 134 (38,5%) e 159 (45,7%) amostras apresentaram-se dentro dos padrões microbiológicos e físico-químicos, respectivamente, segundo a legislação em vigor. Salmonella sp. não foi detectada nas amostras analisadas. Recomenda-se a implantação de programas de boas práticas de fabricação e análise de perigos e pontos críticos de controle para assegurar a inocuidade deste produto.

          Translated abstract

          Nowadays, there are 17 mini-milk processing plants in the state of Alagoas attending the "Fome Zero Program". Microbial and physico-chemical qualities of their products are being monitored at the Food Microbiology Laboratory of the Federal University of Alagoas. Three hundred and forty eight samples of type C pasteurized milk were analyzed for coliforms at 35 and 45 °C; mesophilic and psycrotrophic bacteria, as well as for the presence of Salmonella, according to the methodologies proposed by the Ministry of Agriculture. Physico-chemical analysis parameters (acidity; stability to alcohol; density at 15 °C; freezing point; fat and protein contents and fat-free dry extract) were conducted according to the conventional methodology (Adolfo Lutz Institute). Only fat and protein contents were evaluated by using the Ekomilk apparatus. The results showed that 134 (38.5%) and 159 (45.7%) of the samples were in accordance with the parameters officially established by the Ministry of Agriculture (IN51/2002) for microbiological and physico-chemical parameters, respectively. Salmonella was not detected in the samples. Implementation of good manufacturing practices and HACCP programs will help improving the safety of this product.

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          Instrução Normativa nº 62, de 26 de agosto de 2003. Oficializa os Métodos Analíticos Oficiais para Análises Microbiológicas para Controle de Produtos de Origem Animal e Água

          (2003)
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            Métodos físico-químicos para análise de alimento

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              Isolation and serological identification of enteropathogenic Escherichia coli in pasteurized milk in Brazil

              OBJECTIVE: To evaluate the microbiological quality of pasteurized milk commercialized in Rio de Janeiro, Brazil, and determine serologically enteropathogenic Escherichia coli (EPEC) strains in E. coli isolates obtained from milk samples. METHODS: Ninety samples of pasteurized milk -- types B and C -- of three different commercial brands, purchased in supermarkets and bakeries in Rio de Janeiro, were examined. The amount of total and fecal coliform bacteria was estimated using the Most Probable Number technique. Mesophilic, psychrotrophic, and thermoduric microorganism counts were determined by the Standard Plate Count technique. Isolation and identification of E. coli were carried out using conventional physiological tests. Commercial antisera were used for serological characterization of EPEC. RESULTS: The three milk brands analyzed revealed bacterial counts above the regulated values of the Brazilian government. It was found that among 208 strains of E. coli isolated, 46 (22.1%) were serologically classified as EPEC. The most common EPEC serogroup was O55 (15.2%). CONCLUSIONS: Though recent studies on virulence factors indicate that not all strains serologically classified as EPEC are able to attaching/effacing lesion, it is believed that the isolation of EPEC serogroups from pasteurized milk represent a potential risk for children, as well as an indicative of the presence of other enteropathogens.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                cta
                Food Science and Technology (Campinas)
                Food Sci. Technol (Campinas)
                Sociedade Brasileira de Ciência e Tecnologia de Alimentos (Campinas )
                1678-457X
                March 2008
                : 28
                : 1
                : 226-230
                Affiliations
                [1 ] Universidade Federal de Alagoas Brazil
                Article
                S0101-20612008000100032
                10.1590/S0101-20612008000100032
                90c6ac02-48ef-4605-83ad-465c9117f990

                http://creativecommons.org/licenses/by/4.0/

                History
                Product

                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0101-2061&lng=en
                Categories
                FOOD SCIENCE & TECHNOLOGY

                Food science & Technology
                pasteurized milk,microbiological quality,physico-chemical characterization,leite pasteurizado,qualidade microbiológica,físico-química

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