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Quantitative detection of gold nanoparticles on individual, unstained cancer cells by scanning electron microscopy.

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      Abstract

      Gold nanoparticles are rapidly emerging for use in biomedical applications. Characterization of the interaction and delivery of nanoparticles to cells through microscopy is important. Scanning electron microscopes have the intrinsic resolution to visualize gold nanoparticles on cells. A novel sample preparation protocol was developed to enable imaging of cells and gold nanoparticles with a conventional below lens scanning electron microscopes. The negative influence of 'charging' on the quality of scanning electron microscopes' images could be limited by deposition of biological cells on a conductive (gold) surface. The novel protocol enabled high-resolution scanning electron microscopes' imaging of small clusters and individual gold nanoparticles on uncoated cell surfaces. Gold nanoparticles could be counted on cancer cells with automated routines.

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      Affiliations
      [1 ] Medical Cell BioPhysics, MIRA Institute for Biomedical Technology and Technical Medicine, University of Twente, the Netherlands.
      Journal
      J Microsc
      Journal of microscopy
      Wiley
      1365-2818
      0022-2720
      Nov 2011
      : 244
      : 2
      21801179
      10.1111/j.1365-2818.2011.03528.x

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