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Gold nanoparticles are rapidly emerging for use in biomedical applications. Characterization
of the interaction and delivery of nanoparticles to cells through microscopy is important.
Scanning electron microscopes have the intrinsic resolution to visualize gold nanoparticles
on cells. A novel sample preparation protocol was developed to enable imaging of cells
and gold nanoparticles with a conventional below lens scanning electron microscopes.
The negative influence of 'charging' on the quality of scanning electron microscopes'
images could be limited by deposition of biological cells on a conductive (gold) surface.
The novel protocol enabled high-resolution scanning electron microscopes' imaging
of small clusters and individual gold nanoparticles on uncoated cell surfaces. Gold
nanoparticles could be counted on cancer cells with automated routines.