There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
We report on the re-examination of nine Australian isolates of Actinobacillus pleuropneumoniae
that have been previously assigned to serovar 12. In the ring precipitation test,
none of the nine isolates reacted with antisera to serovars 1-14 of A. pleuropneumoniae.
Antiserum prepared against one of the Australian isolates gave no reaction with any
of the 14 recognised serovar reference strains, except serovar 7. This reaction of
the HS143 antiserum with serovar 7 antigen could be removed by adsorption with serovar
7 antigen. The adsorbed antiserum remained reactive with HS143 and the other eight
Australian isolates. The nine Australian isolates were all shown to express ApxII
and ApxIII, found in serovars 2, 4, 6 and 8, as well as the 42kDa outer membrane protein
found in all serovars of A. pleuropneumoniae. The nine Australian isolates were found
to possess the following toxin associated genes apxIBD, apxIICA, apxIIICA, apxIIIBD
and apxIVA. The toxin gene profile of the Australian isolates is typical of A. pleuropneumoniae
serovars 2, 4, 6 and 8. On the basis of the serological characterisation results and
the toxin gene profiles, we propose that these isolates represent a new serovar of
A. pleuropneumoniae--serovar 15--with HS143 being the reference strain for the new
serovar.