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      Contribution on the study of Isospora hemidactyli Carini, 1936 and a report of an adeleid pseudoparasite of the house gecko Hemidactylus mabouia, from the Rio de Janeiro Metropolitan Region, Brazil Translated title: Contribuição para o estudo de Isospora hemidactyli Carini, 1936 e relato de um pseudoparasita adelídeo da lagartixa doméstica Hemidactylus mabouia da região Metropolitana do Rio de Janeiro, Brasil

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          Abstract

          A description of the coccidium Isospora hemidactyli from the house gecko Hemidactylus mabouia, a very common at dwellings in Rio de Janeiro Metropolitan Region, was made in this study. Histograms and linear regression were made for this species and determined the homogeneity of these oocysts despite of large range. Besides it, polysporocystid oocysts also were recovered from feces of the H. mabouia house gecko and they were similar to those described previously as the genus Adelina. This species should be parasitizing an invertebrate ingested by house gecko, and for that reason, is a pseudoparasite. Oocysts of I. hemidactyli were subspherical to ellipsoidal, 24.4 × 22.3μm, with single-layered wall and one polar granule. Sporocysts were subspherical to ellipsoidal, 11.8 × 9.8μm with Stieda and substieda bodies, residuum and sporozoites with refractile body. Oocysts of the pseudoparasite Adelina sp. were ellipsoidal, 36.3 × 30.9μm, with bi-layered wall and without micropyle, residuum and polar granule. Eight to 15 sporocysts were presents and were subspherical to broadly ellipsoidal, 12.4 × 11.2μm. Stieda and substieda bodies were absent. Sporozoites present refractile bodies at both ends.

          Translated abstract

          Uma descrição do coccídio Isospora hemidactyli da lagartixa doméstica Hemidactylus mabouia, muito comum em residências da região metropolotana do Rio de Janeiro, foi feita neste estudo. Os histogramas e a regressão linear para esta espécie confirmaram a homogeneidade de seus oocistos apesar da grande amplitude de variação. Além disto, oocistos polisporocísticos também foram recuperados das fezes de H. mabouia e foram semelhantes aos descritos anteriormente no gênero Adelina. Esta espécie devia estar parasitando um invertebrado ingerido pela lagartixa doméstica, e por essa razão, é um pseudoparasita. Oocistos de I. hemidactyli foram sub-esféricos a elipsóides, 24,4 × 22,3μm, com parede simples e um grânulo polar. Os esporocistos foram subesféricos a elipsóides, 11,8 × 9,8μm com corpos de Stieda e substieda, resíduo e esporozoítas com corpo refráctil. Oocistos do pseudoparasita Adelina sp. foram elipsóides, 36,3 × 30,9μm, com parede dupla e sem micrópila, resíduo e grânulo polar. Oito a 15 esporocistos estavam presentes e foram subesféricos a elipsóides, 12,4 × 11,2μm. Os corpos de Stieda e substieda estavam ausentes. Os esporozoítas apresentam corpos refrácteis em ambas as extremidades.

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          Estatística Aplicada a Experimentação Animal

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            A guideline for the preparation of species descriptions in the Eimeriidae.

            Members of the suborder Eimeriina (phylum Apicomplexa: class Sporozoea: order Eucoccidiorida) have complex 1 or 2 host life cycles that involve endogenous development in the tissues of vertebrates or invertebrates and exogenous development in an oocyst, usually outside the host(s). Because tissue stages are logistically difficult or even impossible to obtain in natural (wild) host-parasite systems, the vast majority (> 98%) of species in this parasite complex are known only from the structure of their sporulated oocyst. Unfortunately, the quality of these species descriptions is uneven because no guidelines are available for workers in the field to follow. Here we propose a specific set of guidelines for the preparation of species descriptions of coccidia based predominently on the structure of the sporulated oocyst, because the oocyst is the most readily available stage in the life cycle. In addition, we emphasize that ancillary data be incorporated whenever possible with the species description; these data may include, but are not limited to, ecological parameters, prevalence, seasonal data, and the deposition of both host symbiotypes and parasite hepantotypes (= phototypes) into accredited musecums so that accurate identification of both host and parasite material can be assured in perpetuity. And finally, if oocysts are collected in pure suspension, that is, if only one coccidian species (morphotype) is present in the sample, then some oocysts should be saved in 70% ethanol and archived in an accredited museum in the event that future workers might wish to amplify and, later, sequence the parasite's DNA.
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              Epidemiology of protozoan infections: The coccidia

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                Author and article information

                Journal
                rbpv
                Revista Brasileira de Parasitologia Veterinária
                Rev. Bras. Parasitol. Vet.
                Colégio Brasileiro de Parasitologia Veterinária (Jaboticabal, SP, Brazil )
                0103-846X
                1984-2961
                September 2008
                : 17
                : 3
                : 150-154
                Affiliations
                [01] Seropédica RJ orgnameUniversidade Federal Rural do Rio de Janeiro (UFRRJ) Brasil bertobp@ 123456ufrrj.br
                [03] Seropédica RJ orgnameInstituto de Veterinária orgdiv1Departamento de Parasitologia Animal flausino@ 123456ufrrj.br
                [05] Seropédica RJ orgnameInstituto de Veterinária orgdiv1Departamento de Parasitologia Animal lopescwg@ 123456ufrrj.br
                [04] Seropédica RJ orgnameInstituto de Veterinária orgdiv1Departamento de Parasitologia Animal leira@ 123456ufrrj.br
                [02] Vargem Pequena RJ orgnameUniversidade Estácio de Sá Brasil biolopesbb@ 123456hotmail.com
                Article
                S1984-29612008000300006 S1984-2961(08)01700306
                10.1590/S1984-29612008000300006
                987dad25-7d7a-4709-b410-159152c24a6e

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 23 September 2008
                : 03 June 2008
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 16, Pages: 5
                Product

                SciELO Brazil

                Categories
                Articles

                Adelina,oocistos esporulados,Adelidae,sporulated oocysts,Coccidia,Eimeriidae,Coccídios

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