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      Site U1492: Expedition 366

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          Abstract

          Site U1492 is located on the southwestern rim of the summit of Yinazao Seamount (informally known as Blue Moon Seamount) along the extension of an inferred northeast-trending normal fault where there was evidence for interstitial water discharge. Three boreholes were cored to recover materials from the area of the hypothesized conduit. Holes U1492A, U1492B, and U1492C are located 200 m north, 100 m north, and 25 m south, respectively, of Hole U1492D, the site identified to have the highest interstitial water discharge rate in precruise surveys. Screened casing was emplaced in Hole U1492D, and the hole was revisited late in the expedition to seal the base of the casing, leaving a cased/screened borehole ready for a future CORK- Lite deployment.

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          A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types

          A method for the extraction of nucleic acids from a wide range of environmental samples was developed. This method consists of several modules, which can be individually modified to maximize yields in extractions of DNA and RNA or separations of DNA pools. Modules were designed based on elaborate tests, in which permutations of all nucleic acid extraction steps were compared. The final modular protocol is suitable for extractions from igneous rock, air, water, and sediments. Sediments range from high-biomass, organic rich coastal samples to samples from the most oligotrophic region of the world's oceans and the deepest borehole ever studied by scientific ocean drilling. Extraction yields of DNA and RNA are higher than with widely used commercial kits, indicating an advantage to optimizing extraction procedures to match specific sample characteristics. The ability to separate soluble extracellular DNA pools without cell lysis from intracellular and particle-complexed DNA pools may enable new insights into the cycling and preservation of DNA in environmental samples in the future. A general protocol is outlined, along with recommendations for optimizing this general protocol for specific sample types and research goals.
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            Alteration of the oceanic crust: Implications for geochemical cycles of lithium and boron

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              Chemistry of springs across the Mariana forearc shows progressive devolatilization of the subducting plate

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                Author and article information

                Journal
                10.14379/iodp.proc.366.2018
                Proceedings of the International Ocean Discovery Program
                International Ocean Discovery Program
                2377-3189
                07 February 2018
                Article
                10.14379/iodp.proc.366.105.2018
                9deb674d-2e37-4eb2-96c4-df121a4c0048

                This work is licensed under a Creative Commons Attribution 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

                History

                Earth & Environmental sciences,Oceanography & Hydrology,Geophysics,Chemistry,Geosciences

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