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      Surface exposure of phosphatidylserine increases calcium oxalate crystal attachment to IMCD cells.

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          Abstract

          The development of urolithiasis is a multifaceted process, starting at urine supersaturation and ending with the formation of mature renal calculi. The retention of microcrystals by the urothelial cell membrane is a critical event in the process. The current study examines calcium oxalate monohydrate (COM) crystal attachment to inner medullary collecting duct (IMCD) cells following selective changes in cell membrane phospholipid composition. Both primary culture of IMCD cells and a continuous IMCD cell line were used for these studies. Cell membrane composition was selectively altered by either exogenous addition of membrane phospholipids or using membrane lipid scrambling agents. Enrichment with anionic phospholipids was found to greatly increase attachment of crystals to the cells. This increased attachment correlated with the exposure of phosphatidylserine (PS) on the exofacial leaflet of the cell membrane as demonstrated by the use of the membrane scrambling agent A-23187. Furthermore, the increased COM attachment following PS exposure could be blocked by incubating the cells with the PS-specific binding protein, annexin V. These results support the hypothesis that exposure of PS head groups on the papillary epithelial cell surface may mediate stone crystal attachment to the kidney tubule cell epithelium in the renal papilla, possibly as an initiating event in urolithiasis.

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          Author and article information

          Journal
          Am. J. Physiol.
          The American journal of physiology
          0002-9513
          0002-9513
          Jan 1997
          : 272
          : 1 Pt 2
          Affiliations
          [1 ] Department of Medicine, Medical College of Wisconsin, Milwaukee 53295, USA.
          Article
          10.1152/ajprenal.1997.272.1.F55
          9039049
          9e4a3185-809d-4ff7-a305-1c8ed2c91123
          History

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