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      Biochemical changes in hybrid pumpkin seeds at different stages of maturation Translated title: Alterações bioquímicas em sementes híbridas de abóbora em diferentes estádios de maturação

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          Abstract

          ABSTRACT This study aimed to evaluate biochemical changes in seeds of the pumpkin hybrid, 'Jabras', from fruit harvested at different stages of maturation (15, 30, 45, 60 and 75 days after anthesis). Thirty fruit were harvested at each stage, with the seeds from 15 of the fruit being extracted immediately. The remaining 15 were stored for twenty days in plastic boxes and the seeds extracted after this period. After processing and drying the seeds, the following were determined: moisture content, germination, first count and antioxidant enzyme activity (peroxidase, ascorbate peroxidase, catalase and superoxide dismutase). Seeds from the fruit harvested at 30 DAA displayed low values for germination and vigour and high antioxidant enzyme activity, indicating that they were immature and that drying possibly caused damage to the system of cell membranes. The results obtained in this study demonstrated that analysis related to changes in the activity of enzymes during development and maturation of the seeds was effective in evaluating the physiological and biochemical changes in pumpkin seeds of the 'Jabras' cultivar.

          Translated abstract

          RESUMO O presente estudo teve como objetivo avaliar alterações bioquímicas em sementes do híbrido de abóbora 'Jabras', oriundas de frutos colhidos em diferentes estádios de maturação (15; 30; 45; 60 e 75 dias após a antese), sendo que, em cada época, foram colhidos trinta frutos: quinze frutos tiveram suas sementes extraídas imediatamente e os outros quinze foram armazenados por vinte dias em caixas plásticas e, somente após esse período, tiveram suas sementes extraídas. Após o beneficiamento e secagem das sementes, foram realizadas as seguintes determinações: grau de umidade, germinação, primeira contagem e atividade de enzimas antioxidantes (peroxidase, ascorbato peroxidase, catalase e superóxido dismutase). Sementes provenientes de frutos colhidos até 30 DAA apresentaram baixa germinação e vigor e elevada atividade de enzimas antioxidantes, demonstrando que as mesmas encontravam-se imaturas e que a secagem possivelmente, provocou danos ao sistema de membranas celulares. O resultado obtido neste estudo demonstrou que a análise relacionada às alterações na atividade de enzimas durante o desenvolvimento e maturação das sementes foi eficiente para avaliar as alterações fisiológicas e bioquímicas em sementes de abóbora, cultivar 'Jabras'.

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          Regraspara análise de sementes

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            What is stress? Concepts, definitions and applications in seed science.

            'Stresses' that impact upon seeds can affect plant reproduction and productivity, and, hence, agriculture and biodiversity. In the absence of a clear definition of plant stress, we relate concepts from physics, medicine and psychology to stresses that are specific to seeds. Potential 'eustresses' that enhance function and 'distresses' that have harmful effects are considered in relation to the seed life cycle. Taking a triphasic biomedical stress concept published in 1936, the 'General Adaptation Syndrome', to the molecular level, the 'alarm' response is defined by post-translational modifications and stress signalling through cross-talk between reactive oxygen and nitrogen species, and seed hormones, that result in modifications to the transcriptome. Protection, repair, acclimation and adaptation are viewed as the 'building blocks' of the 'resistance' response, which, in seeds, are the basis for their longevity over centuries. When protection and repair mechanisms eventually fail, depending on dose and time of exposure to stress, cell death and, ultimately, seed death are the result, corresponding to 'exhaustion'. This proposed seed stress concept may have wider applicability to plants in general. © The Authors (2010). Journal compilation © New Phytologist Trust (2010).
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              Production of mice through intracytoplasmic injection of sperm or spermatogenic cells

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rca
                Revista Ciência Agronômica
                Rev. Ciênc. Agron.
                Universidade Federal do Ceará (Fortaleza, CE, Brazil )
                0045-6888
                1806-6690
                July 2017
                : 48
                : 3
                : 558-564
                Affiliations
                [2] Pelotas Rio Grande do Sul orgnameUniversidade Federal de Pelotas orgdiv1Departamento de Ciência e Tecnologia de Sementes Brazil patybio55yahoo.com.br, acbarros@ 123456ufpel.edu.br
                [4] Brasília Distrito Federal orgnameEmbrapa Hortaliças Brazil warley.nascimento@ 123456embrapa.br
                [3] Viçosa Minas Gerais orgnameUniversidade Federal de Viçosa orgdiv1Departamento de Fitotecnia Brazil dcdiasufv.br, marcelosekita@ 123456yahoo.com.br
                Article
                S1806-66902017000300558
                10.5935/1806-6690.20170065
                a480867b-254d-4b68-8164-110ec9953722

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 08 January 2016
                : 16 August 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 18, Pages: 7
                Product

                SciELO Brazil


                Cucurbita maxima,Cucurbita moschata,Enzimas antioxidantes,Antioxidant enzymes

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