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      Tomato spotted wilt virus (TS WV), weeds and thrip vectors in the tomato (Solanum lycopersicum L.) in the Andean region of Cundinamarca (Colombia) Translated title: Tomato spotted wilt virus (TSWV), malezas y vectores de trips en el tomate (Solanum lycopersicum L.) en la región andina de Cundinamarca (Colombia)

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          Abstract

          The presence and distribution of the TSWV, weeds and thrip vectors in major tomato producing areas in the Andean department of Cundinamarca (Oriente, Sumapaz and Ubate provinces) were assessed with the DAS ELISA technique, evaluating the presence of the TSWV in tomato tissue, associated thrips and weeds. High incidences were observed in different provinces of the Andean department of Cundinamarca. The average viral incidence reached 23.3% in Sumapaz, 19.4% in Oriente and 4% in Ubate. The symptoms observed were: brown spots and concentric rings in the leaf area, stems and fruits; browning and spotting in the flower; and wilting in the leaves, stems and flowers. The thrip species with the highest presence were Frankliniella occidentalis, followed by Thrips palmi and Thrips tabaci. We determined the important role of weeds as inoculum sources and vector reservoirs for the species Emilia sonchifolia and Amaranthus dubius

          Translated abstract

          La presencia y distribución de TSWV, arvenses y los trips vectores en las principales zonas productoras de tomate en la región andina del departamento de Cundinamarca (provincias de Oriente, Sumapaz y Ubaté), se confirmó mediante la técnica DAS ELISA , se evaluó la presencia del virus TSWV en tejido de tomate, arvenses y trips asociados. Se observaron incidencias altas en diferentes provincias de la región andina del departamento de Cundinamarca. La incidencia viral alcanzó promedios de 23,3% en el Sumapaz, el 19,4% en Oriente y el 4% en Ubaté. Los síntomas característicos observados correspondieron a manchas de color café y anillos concéntricos en el área foliar, tallos y frutos, bronceado, manchas en flor y marchitez en hojas, tallos y flores. Las especies de trips con mayor presencia fueron Frankliniella occidentalis, seguida de Thrips palmi y Thrips tabaci. Se determinó el importante papel de las arvenses como fuente de inóculo y reservorio de vectores en las especies Emilia sonchifolia y Amaranthus dubius

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          Most cited references49

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          Tomato spotted wilt virus Infection Improves Host Suitability for Its Vector Frankliniella occidentalis.

          ABSTRACT The effect of Tomato spotted wilt virus (TSWV) infection on plant attractiveness for the western flower thrips (Frankliniella occidentalis) was studied. Significantly more thrips were recovered on infected than were recovered on noninfected pepper (Capsicum annuum) plants in different preference tests. In addition, more offspring were produced on the virus-infected pepper plants, and this effect also was found for TSWV-infected Datura stramonium. Thrips behavior was minimally influenced by TSWV-infection of host plants with only a slight preference for feeding on infected plants. Offspring development was positively affected since larvae hatched earlier from eggs and subsequently pupated faster on TSWV-infected plants. These results show a mutualistic relationship between F. occidentalis and TSWV.
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            Role of Weeds in the Incidence of Virus Diseases

            J E Duffus (1971)
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              Multiplication of tomato spotted wilt virus in its insect vector, Frankliniella occidentalis.

              The accumulation of two proteins, the nucleocapsid (N) protein and a non-structural (NSs) protein both encoded by the S RNA of tomato spotted wilt virus (TSWV), was followed in larvae during development and in adults of Frankliniella occidentalis after ingesting the virus for short periods on infected plants. The amounts of both proteins increased, as shown by ELISA and Western blot analysis, within 2 days above the levels ingested, indicating multiplication of TSWV in these insects. Accumulation of these proteins and of virus particles was further confirmed by in situ immunolabelling of the salivary glands and other tissues of adult thrips. The accumulation of large amounts of N and NSs protein, the occurrence of several vesicles with virus particles in the salivary glands and the massive numbers of virus particles in the salivary gland ducts demonstrate that the salivary glands are a major site of TSWV replication. The occurrence of virus particles in the salivary vesicles is indicative of the involvement of the Golgi apparatus in the maturation of the virus particles and its transport to the salivary ducts.
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                Author and article information

                Journal
                agc
                Agronomía Colombiana
                Agron. colomb.
                Universidad Nacional de Colombia, Facultad de Agronomía (Bogotá, Distrito Capital, Colombia )
                0120-9965
                April 2013
                : 31
                : 1
                : 58-67
                Affiliations
                [03] orgnameInstituto Politécnico Nacional (CEPR OBI)
                [02] orgnameUniversidad de los Andes orgdiv1Faculty of Sciences
                [05] orgnameUniversidad Nacional de Colombia orgdiv1Faculty of Agronomy
                [01] orgnameInstituto Colombiano Agropecuario (ICA) everth.ebratt@ 123456ica.gov.co
                [04] orgnameCorporación Universitaria Minuto de Dios
                Article
                S0120-99652013000100007 S0120-9965(13)03100107
                a78248e4-42af-4b33-a827-78f954a689d8

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 29 March 2012
                : 10 March 2012
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 54, Pages: 10
                Product

                SciELO Colombia

                Self URI: Full text available only in PDF format (EN)
                Categories
                Protection of cultivations

                weeds,Frankliniella occidentalis,Thrips palmi,Thrips tabaci,arvenses,Tospovirus

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