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      Primera detección de Candidatus Rickettsia colombianensi en el departamento del Meta, Colombia Translated title: First detection of Candidatus Rickettsia colombianensi in the State of Meta, Colombia

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          Abstract

          RESUMEN Introducción: Las Rickettsias son un género de bacterias Gram negativas intracelulares obligadas causantes de muchas epidemias en el mundo y son transmitidas principalmente por garrapatas, pulgas, piojos y ácaros. Las rickettsiosis son enfermedades infecciosas reemergentes sin vigilancia epidemiológica en Colombia. Hasta hace pocos años Rickettsia rickettsi era la única Rickettsia transmitida por garrapatas presente en América; sin embargo, nuevas especies están siendo descritas, y aunque su patogenicidad no ha sido confirmada pueden ser consideradas patógenos potenciales. Objetivo: Determinar la presencia de rickettsias en garrapatas del departamento del Meta, Colombia. Material y Métodos: Se realizó un estudio descriptivo de corte transversal, fueron colectadas garrapatas y después de su identificación taxonómica, los genes gltA, ompA y ompB fueron amplificados y secuenciados. Resultados: Se obtuvieron un total de 169 grupos a los que se les realizó PCR de los cuales 2 grupos amplificaron para los genes gltA, ompA y ompB. Conclusiones: Los resultados demuestran la presencia de Rickettsia spp en garrapatas del departamento del Meta.

          Translated abstract

          ABSTRACT Introduction: Rickettsia is a genus of Gram-negative obligate intracellular bacteria that cause several epidemics around the world and are transmitted mainly by ticks, fleas, lice and mites. Rickettsiosis is a re-emergent infectious disease without epidemiological surveillance in Colombia. Until few years ago, Rickettsia rickettsi was the only tick-borne Rickettsioses in America; however, new species are being described, and although their pathogenicity has not been confirmed, they should be considered as potential pathogens. Objective: The aim of this study was to determine the presence of rickettsial agents in ticks in the state of Meta, Colombia. Material and Methods: A descriptive cross-sectional study was conducted; ticks were collected and, after their taxonomic identification, gltA,ompAand ompB genes were amplified and sequenced. Results: A total of 169 pools were obtained to which PCR were carried out, of which 2 PCR were amplified for the gltA, ompA and ompB genes. Conclusions: These results demonstrate the presence of Rickettsia spp in ticks in the State of Meta.

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          Most cited references18

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          MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.

          We announce the release of an advanced version of the Molecular Evolutionary Genetics Analysis (MEGA) software, which currently contains facilities for building sequence alignments, inferring phylogenetic histories, and conducting molecular evolutionary analysis. In version 6.0, MEGA now enables the inference of timetrees, as it implements the RelTime method for estimating divergence times for all branching points in a phylogeny. A new Timetree Wizard in MEGA6 facilitates this timetree inference by providing a graphical user interface (GUI) to specify the phylogeny and calibration constraints step-by-step. This version also contains enhanced algorithms to search for the optimal trees under evolutionary criteria and implements a more advanced memory management that can double the size of sequence data sets to which MEGA can be applied. Both GUI and command-line versions of MEGA6 can be downloaded from www.megasoftware.net free of charge.
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            Rickettsia parkeri: a newly recognized cause of spotted fever rickettsiosis in the United States.

            Ticks, including many that bite humans, are hosts to several obligate intracellular bacteria in the spotted fever group (SFG) of the genus Rickettsia. Only Rickettsia rickettsii, the agent of Rocky Mountain spotted fever, has been definitively associated with disease in humans in the United States. Herein we describe disease in a human caused by Rickettsia parkeri, an SFG rickettsia first identified >60 years ago in Gulf Coast ticks (Amblyomma maculatum) collected from the southern United States. Confirmation of the infection was accomplished using serological testing, immunohistochemical staining, cell culture isolation, and molecular methods. Application of specific laboratory assays to clinical specimens obtained from patients with febrile, eschar-associated illnesses following a tick bite may identify additional cases of R. parkeri rickettsiosis and possibly other novel SFG rickettsioses in the United States.
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              Rickettsia Species Infecting Amblyomma cooperi Ticks from an Area in the State of Sao Paulo, Brazil, Where Brazilian Spotted Fever Is Endemic

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                Author and article information

                Journal
                rhcm
                Revista Habanera de Ciencias Médicas
                Rev haban cienc méd
                Universidad de Ciencias Médicas de la Habana (La Habana, , Cuba )
                1729-519X
                June 2019
                : 18
                : 3
                : 487-499
                Affiliations
                [1] Meta orgnameUniversidad Cooperativa de Colombia orgdiv1Facultad de Medicina, sede Villavicencio Colombia
                [2] Montería Córdoba orgnameUniversidad de Córdoba orgdiv1Instituto de investigaciones biológicas del trópico Colombia
                [3] La Habana La Habana orgnameInstituto de Medicina Tropical Pedro Kourí orgdiv1Laboratorio Nacional de Referencia de Espiroquetas y Brucelas Cuba
                Article
                S1729-519X2019000300487 S1729-519X(19)01800300487
                a9eff998-747a-4a51-b9d1-78211075402a

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 27 April 2019
                : 29 January 2019
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 23, Pages: 13
                Product

                SciELO Cuba

                Categories
                CIENCIAS EPIDEMIOLÓGICAS Y SALUBRISTAS

                Colombia,Ticks,Garrapatas,IFI,Rickettsiosis,Candidatus Rickettsia colombianensi,PCR

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