Blog
About

  • Record: found
  • Abstract: found
  • Article: found
Is Open Access

Carbon Monoxide Is Involved in Hydrogen Gas-Induced Adventitious Root Development in Cucumber under Simulated Drought Stress

Read this article at

Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      Hydrogen gas (H 2) and carbon monoxide (CO) are involved in plant growth and developmental processes and may induce plant tolerance to several stresses. However, the independent roles and interaction effect of H 2 and CO in adventitious root development under drought conditions have still not received the needed research attention. We hypothesize that there exists crosstalk between H 2 and CO during adventitious root development under drought stress. The results of our current study revealed that 50% (v/v) hydrogen-rich water (HRW), 500 μM Hemin (the CO donor) and 30% (w/v) CO aqueous solution apparently promoted the development of adventitious roots in cucumber explants ( Cucumis Sativus L.) under drought stress. H 2 and CO increased relative water content (RWC), leaf chlorophyll content (chlorophyll a, b, and a+b), and chlorophyll fluorescence parameters [photochemical efficiency of photosystem II (PSII), PSII actual photochemical efficiency and photochemical quench coefficient] under drought condition. When the CO scavenger hemoglobin (Hb) or zinc protoporphyrin IX (ZnPPIX) was added to HRW/CO aqueous solution, the positive effect of HRW/CO aqueous solution on RWC, leaf chlorophyll content, and chlorophyll fluorescence parameters were reversed. Additionally, superoxide dismutases, peroxidase, catalase, and ascorbate peroxidase was significantly increased in the explants treated with HRW and CO aqueous solution under drought stress, thus alleviating oxidative damage, as indicated by decreases in thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H 2O 2), and superoxide radical (O 2 -) levels. H 2 and CO also improved the levels of water soluble carbohydrate, total soluble protein, and proline content. However, the above CO/H 2-mediated effects were reversed by CO scavenger Hb or CO specific synthetic inhibitor ZnPPIX. Therefore, CO may be involved in H 2-induced adventitious rooting under drought stress and alleviate oxidative damage by enhancing RWC, leaf chlorophyll content, chlorophyll fluorescence parameters, metabolic constituent content, activating anti-oxidant enzymes and reducing TBARS, O 2 -, and H 2O 2 levels.

      Related collections

      Most cited references 53

      • Record: found
      • Abstract: not found
      • Article: not found

      A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

        Bookmark
        • Record: found
        • Abstract: not found
        • Article: not found

        Rapid determination of free proline for water-stress studies

          Bookmark
          • Record: found
          • Abstract: not found
          • Article: not found

          COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

           Tal I. Arnon,  D Arnon (1948)
            Bookmark

            Author and article information

            Affiliations
            College of Horticulture, Gansu Agricultural University Lanzhou, China
            Author notes

            Edited by: Ruth Grene, Virginia Tech, USA

            Reviewed by: Erik T. Nilsen, Virginia Tech, USA; Bishal Gole Tamang, University of Minnesota, USA

            *Correspondence: Weibiao Liao, liaowb@ 123456gsau.edu.cn

            This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science

            Contributors
            Journal
            Front Plant Sci
            Front Plant Sci
            Front. Plant Sci.
            Frontiers in Plant Science
            Frontiers Media S.A.
            1664-462X
            07 February 2017
            2017
            : 8
            5293791 10.3389/fpls.2017.00128
            Copyright © 2017 Chen, Wang, Hu, Liao, Dawuda and Li.

            This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

            Counts
            Figures: 7, Tables: 1, Equations: 0, References: 58, Pages: 16, Words: 0
            Funding
            Funded by: National Natural Science Foundation of China 10.13039/501100001809
            Award ID: 31160398
            Award ID: 31560563
            Funded by: Natural Science Foundation of Gansu Province 10.13039/501100004775
            Award ID: 1606RJZA073
            Award ID: 1606RJZA077
            Award ID: 1308RJZA179
            Award ID: 1308RJZA262
            Categories
            Plant Science
            Original Research

            Comments

            Comment on this article