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      Carbon Monoxide Is Involved in Hydrogen Gas-Induced Adventitious Root Development in Cucumber under Simulated Drought Stress

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          Abstract

          Hydrogen gas (H 2) and carbon monoxide (CO) are involved in plant growth and developmental processes and may induce plant tolerance to several stresses. However, the independent roles and interaction effect of H 2 and CO in adventitious root development under drought conditions have still not received the needed research attention. We hypothesize that there exists crosstalk between H 2 and CO during adventitious root development under drought stress. The results of our current study revealed that 50% (v/v) hydrogen-rich water (HRW), 500 μM Hemin (the CO donor) and 30% (w/v) CO aqueous solution apparently promoted the development of adventitious roots in cucumber explants ( Cucumis Sativus L.) under drought stress. H 2 and CO increased relative water content (RWC), leaf chlorophyll content (chlorophyll a, b, and a+b), and chlorophyll fluorescence parameters [photochemical efficiency of photosystem II (PSII), PSII actual photochemical efficiency and photochemical quench coefficient] under drought condition. When the CO scavenger hemoglobin (Hb) or zinc protoporphyrin IX (ZnPPIX) was added to HRW/CO aqueous solution, the positive effect of HRW/CO aqueous solution on RWC, leaf chlorophyll content, and chlorophyll fluorescence parameters were reversed. Additionally, superoxide dismutases, peroxidase, catalase, and ascorbate peroxidase was significantly increased in the explants treated with HRW and CO aqueous solution under drought stress, thus alleviating oxidative damage, as indicated by decreases in thiobarbituric acid reactive substances (TBARS), hydrogen peroxide (H 2O 2), and superoxide radical (O 2 -) levels. H 2 and CO also improved the levels of water soluble carbohydrate, total soluble protein, and proline content. However, the above CO/H 2-mediated effects were reversed by CO scavenger Hb or CO specific synthetic inhibitor ZnPPIX. Therefore, CO may be involved in H 2-induced adventitious rooting under drought stress and alleviate oxidative damage by enhancing RWC, leaf chlorophyll content, chlorophyll fluorescence parameters, metabolic constituent content, activating anti-oxidant enzymes and reducing TBARS, O 2 -, and H 2O 2 levels.

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          Most cited references 53

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          A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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            Rapid determination of free proline for water-stress studies

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              COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.

               Tal I. Arnon,  D Arnon (1948)
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                Author and article information

                Contributors
                Journal
                Front Plant Sci
                Front Plant Sci
                Front. Plant Sci.
                Frontiers in Plant Science
                Frontiers Media S.A.
                1664-462X
                07 February 2017
                2017
                : 8
                Affiliations
                College of Horticulture, Gansu Agricultural University Lanzhou, China
                Author notes

                Edited by: Ruth Grene, Virginia Tech, USA

                Reviewed by: Erik T. Nilsen, Virginia Tech, USA; Bishal Gole Tamang, University of Minnesota, USA

                *Correspondence: Weibiao Liao, liaowb@ 123456gsau.edu.cn

                This article was submitted to Plant Abiotic Stress, a section of the journal Frontiers in Plant Science

                10.3389/fpls.2017.00128
                5293791
                Copyright © 2017 Chen, Wang, Hu, Liao, Dawuda and Li.

                This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

                Counts
                Figures: 7, Tables: 1, Equations: 0, References: 58, Pages: 16, Words: 0
                Funding
                Funded by: National Natural Science Foundation of China 10.13039/501100001809
                Award ID: 31160398
                Award ID: 31560563
                Funded by: Natural Science Foundation of Gansu Province 10.13039/501100004775
                Award ID: 1606RJZA073
                Award ID: 1606RJZA077
                Award ID: 1308RJZA179
                Award ID: 1308RJZA262
                Categories
                Plant Science
                Original Research

                Plant science & Botany

                enzyme activity, adventitious rooting, chlorophyll fluorescence, co, hrw, drought

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