Lightwave-reinforced stem cells with enhanced wound healing efficacy

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Abstract

Comprehensive research has led to significant preclinical outcomes in modified human adipose-derived mesenchymal stem cells (hADSCs). Photobiomodulation (PBM), a technique to enhance the cellular capacity of stem cells, has attracted considerable attention owing to its effectiveness and safety. Here, we suggest a red organic light-emitting diode (OLED)-based PBM strategy to augment the therapeutic efficacy of hADSCs. In vitro assessments revealed that hADSCs basked in red OLED light exhibited enhanced angiogenesis, cell adhesion, and migration compared to naïve hADSCs. We demonstrated that the enhancement of cellular capacity was due to an increased level of intracellular reactive oxygen species. Furthermore, accelerated healing and regulated inflammatory response was observed in mice transplanted with red light-basked hADSCs. Overall, our findings suggest that OLED-based PBM may be an easily accessible and attractive approach for tissue regeneration that can be applied to various clinical stem cell therapies.

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Most cited references 96

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In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro.

Chun-Chi Liang, Ann Park, Jun-Lin Guan (2007)

The in vitro scratch assay is an easy, low-cost and well-developed method to measure cell migration in vitro. The basic steps involve creating a "scratch" in a cell monolayer, capturing the images at the beginning and at regular intervals during cell migration to close the scratch, and comparing the images to quantify the migration rate of the cells. Compared to other methods, the in vitro scratch assay is particularly suitable for studies on the effects of cell-matrix and cell-cell interactions on cell migration, mimic cell migration during wound healing in vivo and are compatible with imaging of live cells during migration to monitor intracellular events if desired. Besides monitoring migration of homogenous cell populations, this method has also been adopted to measure migration of individual cells in the leading edge of the scratch. Not taking into account the time for transfection of cells, in vitro scratch assay per se usually takes from several hours to overnight.

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Antibacterial anti-oxidant electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing.

Xin Zhao, Hao Wu, Baolin Guo … (2017)

Injectable self-healing hydrogel dressing with multifunctional properties including anti-infection, anti-oxidative and conductivity promoting wound healing process will be highly desired in wound healing application and its design is still a challenge. We developed a series of injectable conductive self-healed hydrogels based on quaternized chitosan-g-polyaniline (QCSP) and benzaldehyde group functionalized poly(ethylene glycol)-co-poly(glycerol sebacate) (PEGS-FA) as antibacterial, anti-oxidant and electroactive dressing for cutaneous wound healing. These hydrogels presented good self-healing, electroactivity, free radical scavenging capacity, antibacterial activity, adhesiveness, conductivity, swelling ratio, and biocompatibility. Interestingly, the hydrogel with an optimal crosslinker concentration of 1.5 wt% PEGS-FA showed excellent in vivo blood clotting capacity, and it significantly enhanced in vivo wound healing process in a full-thickness skin defect model than quaternized chitosan/PEGS-FA hydrogel and commercial dressing (Tegaderm™ film) by upregulating the gene expression of growth factors including VEGF, EGF and TGF-β and then promoting granulation tissue thickness and collagen deposition. Taken together, the antibacterial electroactive injectable hydrogel dressing prolonged the lifespan of dressing relying on self-healing ability and significantly promoted the in vivo wound healing process attributed to its multifunctional properties, meaning that they are excellent candidates for full-thickness skin wound healing.

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Growth factors and cytokines in wound healing.

Stephan Barrientos, Olivera Stojadinovic, Michael S. Golinko … (2009)

Wound healing is an evolutionarily conserved, complex, multicellular process that, in skin, aims at barrier restoration. This process involves the coordinated efforts of several cell types including keratinocytes, fibroblasts, endothelial cells, macrophages, and platelets. The migration, infiltration, proliferation, and differentiation of these cells will culminate in an inflammatory response, the formation of new tissue and ultimately wound closure. This complex process is executed and regulated by an equally complex signaling network involving numerous growth factors, cytokines and chemokines. Of particular importance is the epidermal growth factor (EGF) family, transforming growth factor beta (TGF-beta) family, fibroblast growth factor (FGF) family, vascular endothelial growth factor (VEGF), granulocyte macrophage colony stimulating factor (GM-CSF), platelet-derived growth factor (PDGF), connective tissue growth factor (CTGF), interleukin (IL) family, and tumor necrosis factor-alpha family. Currently, patients are treated by three growth factors: PDGF-BB, bFGF, and GM-CSF. Only PDGF-BB has successfully completed randomized clinical trials in the Unites States. With gene therapy now in clinical trial and the discovery of biodegradable polymers, fibrin mesh, and human collagen serving as potential delivery systems other growth factors may soon be available to patients. This review will focus on the specific roles of these growth factors and cytokines during the wound healing process.

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Author and article information

Contributors

Yu-Jin Kim: Role: ConceptualizationRole: MethodologyRole: Formal analysisRole: InvestigationRole: Writing original draftRole: Visualization

Hye Ran Jeon: Role: ConceptualizationRole: MethodologyRole: Formal analysisRole: InvestigationRole: Writing original draftRole: Visualization

Sung-Won Kim: Role: MethodologyRole: Investigation

Yeong Hwan Kim: Role: MethodologyRole: Investigation

Gwang-Bum Im: Role: MethodologyRole: Investigation

Jisoo Im: Role: MethodologyRole: Investigation

Soong Ho Um: Role: Writing review editingRole: Funding acquisition

Sung Min Cho: Role: Writing review editingRole: Supervision

Ju-Ro Lee: Role: Writing review editing

Han Young Kim: Role: Writing review editingRole: Funding acquisition

Yoon Ki Joung: Role: Writing review editing

Dong-Ik Kim: Role: ConceptualizationRole: Formal analysisRole: Writing original draftRole: Writing review editingRole: SupervisionRole: Funding acquisition

Suk Ho Bhang:

ORCID: https://orcid.org/0000-0003-3002-0590

Role: ConceptualizationRole: Formal analysisRole: Writing original draftRole: Writing review editingRole: SupervisionRole: Funding acquisition

Journal

Journal ID (nlm-ta): J Tissue Eng

Journal ID (iso-abbrev): J Tissue Eng

Journal ID (publisher-id): TEJ

Journal ID (hwp): sptej

Title: Journal of Tissue Engineering

Publisher: SAGE Publications (Sage UK: London, England )

ISSN (Electronic): 2041-7314

Publication date (Electronic): 18 December 2021

Publication date Collection: Jan-Dec 2021

Volume: 12

Electronic Location Identifier: 20417314211067004

Affiliations

[1 ]School of Chemical Engineering, Sungkyunkwan University, Suwon, Gyeonggi-do, Republic of Korea

[2 ]Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Gangnam-gu, Seoul, Republic of Korea

[3 ]Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul, Republic of Korea

[4 ]Center for Biomaterials, Biomedical Research Institute, Korea Institute of Science and Technology, Seoungbuk-gu, Seoul, Republic of Korea

[5 ]Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon, Gyeonggi, Republic of Korea

[6 ]Division of Bio-Medical Science & Technology, University of Science and Technology, Yuseong-gu, Daejeon, Republic of Korea

Author notes

[*]Dong-Ik Kim, Department of Health Sciences and Technology, SAIHST, Sungkyunkwan University, Gangnam-gu, Seoul 06355, Republic of Korea, Division of Vascular Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Gangnam-gu, Seoul 06351, Republic of Korea. Email: dikim@ 123456skku.edu

[*]Suk Ho Bhang, School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do 16419, Republic of Korea. Email: sukhobhang@ 123456skku.edu

[*]

These authors contributed equally to this work.

Author information

Suk Ho Bhang https://orcid.org/0000-0003-3002-0590

Article

Publisher ID: 10.1177_20417314211067004

DOI: 10.1177/20417314211067004

PMC ID: 8721371

PubMed ID: 34987748

SO-VID: b372d50b-a247-492b-b4ad-b4e0de5f9ccb

License:

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ( https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page ( https://us.sagepub.com/en-us/nam/open-access-at-sage).

History

Date received : 13 October 2021

Date accepted : 29 November 2021

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cover-date January-December 2021

typesetter ts1

ScienceOpen disciplines: Biomedical engineering

Keywords: photobiomodulation,human adipose-derived stem cells,wound healing,angiogenesis

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ScienceOpen disciplines: Biomedical engineering

Keywords: photobiomodulation, human adipose-derived stem cells, wound healing, angiogenesis

Lightwave-reinforced stem cells with enhanced wound healing efficacy

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Abstract

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Stress signalling in stem cells

Most cited references 96

In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro.

Antibacterial anti-oxidant electroactive injectable hydrogel as self-healing wound dressing with hemostasis and adhesiveness for cutaneous wound healing.

Growth factors and cytokines in wound healing.

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