Pathology after combined epicardial and endocardial ablation for ventricular tachycardia in a postmortem heart with hypertrophic cardiomyopathy

Abstract

Introduction KEY TEACHING POINTS • Lesion depth created by radiofrequency catheter ablation is smaller on the epicardium with adipose tissue than on the endocardium. • It seems to be difficult to create transmural lesions even by combined epicardial and endocardial ablation, especially in patients with thickened ventricular walls. • Some approaches such as needle, bipolar, and transcoronary ethanol ablation may be more effective to create transmural lesions. Ventricular arrhythmias in patients with nonischemic cardiomyopathy (NICM) often affect patients’ prognosis. Ventricular tachycardias (VTs) arising from the electrical scar area in hearts of NICM patients could become targets for radiofrequency catheter ablation (RFCA) therapy.1, 2, 3 However, the efficacy of RFCA using the endocardial approach is limited because of the deeper localization of the electrical scar from the intramural to the epicardial side.1, 2, 3 Recently, combined endocardial and epicardial ablation has been used to improve the RFCA outcomes for VT in patients with NICM. However, elimination of the VTs is still challenging,1, 2, 3, 4, 5, 6 especially in patients with hypertrophic cardiomyopathy (HCM).7, 8 In this case report, we evaluated the pathologic features after combined epicardial and endocardial ablation in a postmortem heart from a patient with HCM. Case report The patient was a 73-year-old man who was diagnosed with HCM at the age of 63 years. The left ventricular chamber showed progressive dilation with contractile dysfunction from the age of 67, and the heart was thought to be in an advanced dilated form. In spite of the administration of β-blocker and amiodarone as concomitant medical therapy for heart failure and nonsustained VT, the effect was insufficient, and he was implanted with cardiac resynchronization therapy with defibrillator. The patient was admitted to our hospital because of repetitive drug-resistant VT at the age of 73 years. Echocardiography revealed an aneurysm at the apical portion of the left ventricle (LV), which was accompanied by severe LV dysfunction (left ventricular ejection fraction 21%) and asymmetric septal hypertrophy. On the day of admission, the patient presented with VT storm, was intubated, and underwent assisted cardiopulmonary support because the VTs were resistant to antiarrhythmic drugs and hemodynamically unstable. Coronary angiography showed no significant stenosis in coronary arteries. Under intravenous β-blocker administration, RFCA was performed to control the VT storm. Electrophysiological examination and ablation procedure Most of the clinical VTs in the 12-lead electrocardiogram (ECG) showed a right bundle branch block pattern with slowed initial precordial QRS activation (Figure 1A). We performed RFCA using the epicardial and endocardial approach because the origins of some VTs were thought to be located on the epicardial side based on the 12-lead ECG findings. Steerable catheters were inserted from the right femoral vein and placed in the coronary sinus and the ventricle of interest. The LV endocardium was accessed using the trans-septal approach. Pericardial access was obtained through a subxiphoid and anterior puncture under fluoroscopic guide. We used a 3.5-mm open-irrigatedtip ablation catheter, Thermocool (Biosense Webster, Johnson & Johnson, Diamond Bar, CA), which was also used for mapping. Bipolar voltage maps of the endocardium and epicardium were constructed during sinus rhythm. Arrhythmogenic areas, indicated by low-voltage zones (LVZs), which were defined as <1.5 mV at the endocardium and <1.0 mV at the epicardium, were mainly located on the lateral to posterior wall in the LV endocardium and throughout the entire LV in the epicardium (Figure 2A and B). Six forms of VTs were induced by right ventricular pacing, although clinically documented VTs did not appear (Figure 1B). We performed substrate-based RFCA, which targeted the LVZs and abnormal electrograms such as delayed potentials, fragmented potentials, and/or double potentials in the endocardium and epicardium. Open-irrigated radiofrequency (RF) current was delivered for up to 60 seconds in power-controlled mode as follows: power 30–45 W and irrigation 17–30 mL/min. Total application time of RFCA was about 86 minutes. Clinical course after ablation A VT that was not induced in the electrophysiological study emerged after the RFCA session. The VT was clinically controlled by antiarrhythmic drugs (amiodarone, nifekalant, and lidocaine). The patient, however, died from deterioration of heart failure 49 days after the procedure. A postmortem examination of the heart was performed. Postmortem gross pathologic findings The epicardium of the heart was mildly adhered to the pericardium around the apex of the LV with a thin fibrinous exudate, and this was thought to be an inflammatory reaction after the epicardial ablation procedure. There was no increase in pericardial fluid volume. The heart was significantly enlarged and weighed 820 g. There was rich epicardial fat attached in the atrioventricular and interventricular groove, and it was also widely distributed in the free wall of the LV that roughly corresponded to the voltage map findings (Figure 2B and C). On the LV epicardial surface, RFCA results showed discolored spotty lesions around the LV apex. All 4 chambers of the heart were dilated along the long axis section through the heart. The LV wall showed asymmetric hypertrophy as follows: 27 mm thickness in the ventricular septum, and the papillary muscle was also hypertrophied (Figure 3A). The LV apical wall was thinning with complex endocardial trabeculation, and it showed aneurysmal dilation that was thought to be an arrhythmogenic source. The ablated lesions from the endocardial approach were observed as blackish spots mainly located in and at the border of the aneurysmal lesion. All coronary arteries showed no arteriosclerotic stenosis. Ablated lesion beneath the epicardium and endocardium Figure 3 shows a cross section of the ablated lesions at the border of the LV aneurysm, where both the epicardial and endocardial approach ablation lesions were assessed. The endocardial lesions were clearly defined and were deeper than the epicardial lesions. The cloudy discolored areas were necrotic tissue caused by RF energy, and the adjacent black-colored areas were lesions with hemorrhage caused by damage to intramural small coronary arteries and capillaries. On the epicardial side, the LV apex around the ablated lesions was diffusely covered by fatty tissue, and its thickness was up to 5 mm. The fatty layer was transmurally discolored; however, the change of the myocardium beneath the epicardial fat was limited (Figure 3B). Figure 3C shows the identical histologic sections to Figure 3B, and the original pathologic fibrosis resulting from HCM diffusely extended mainly in the center and epicardial side of the LV wall. The ablation scar reached a depth of 6 mm from the endocardial surface at its deepest site, whereas the ablation scar from the epicardial surface reached only 2 mm beyond the epicardial fat. Between the epicardial and endocardial ablated lesions, there was approximately 1.5 mm of viable myocardial tissue with pathologic fibrosis that escaped the RF energy at the border of the aneurysmal wall, and there were no obvious transmural ablation lesions. In addition, the spared viable myocardium was especially noted on the top of the trabeculi or bridging the myocardial bundles, despite that the deeper sites were ablated. The peripheral area of the ablated sites was replaced with mature fibrous tissue, but the remaining necrotic tissue and hemorrhagic area were still observed at the core of the ablated sites. This histologic discrepancy in the relatively long phase after RF (approximately 6 weeks) may be attributed to the prolonged wound healing process of the intramural hemorrhage and small vessel injuries. 9 Discussion This case provided us with important information by the analysis of pathologic changes after combined epicardial and endocardial ablation with open-irrigated catheter for the first time in a human heart. We focused on the 2 major findings in this heart, as described below. The finding of voltage maps with larger LVZ on the epicardium than that on the endocardium was roughly in accordance with the histopathology of the heart (Figure 3C and Supplemental Figure, available online). We assumed that the LVZ on the epicardium, however, indicated not only original pathologic fibrosis but also the fat on the epicardium. The pathologic evaluation showed that transmural ablation could not be achieved in this case. Even though new strategies such as local abnormal ventricular activity ablation or homogenization of the scar area in electroanatomic mapping have been developed for unstable hemodynamic VTs, efficacy of epicardial ablation is limited in cases like this, which might be attributed to residual arrhythmic substrate, especially in NICM.10, 11 The saline irrigation catheter produces deeper and larger ablation lesions in vivo compared with a non–irrigation catheter. 12 However, despite the use of open-irrigated epicardial ablation, transmural ablation lesions are found in only 55% of cases. 13 Some studies reported that the lesions made by epicardial ablations were affected by the rich adipose tissues in the epicardium. 14 The degree of influence of the epicardial fat thickness on the RF delivery is controversial. Van Huls van Taxis et al 15 reported the clear cut-off of thickness threshold to impair RF delivery as 7 mm. On the other hand, Hong et al 16 reported that the epicardial fat could severely limit the lesion depth created by RF energy, even in the case with the epicardial fat thickness of 2 mm. 16 In this case, ablated scars were not reached deeper from epicardial site than those from endocardial site because of rich adipose tissues. The impaired RF delivery, which was demonstrated by limited ablation lesions from the epicardial surface, might also result from the catheter instability, weak contact force, and/or inadequate RF power on the epicardium. The VT recurrence after RFCA might be also due to inability to map and/or identify critical sites of VTs. We retrospectively examined the electrogram response (R wave reduction of the electrogram) by RF delivery in this case, and compared it between RF delivery from the epicardium and from the endocardium. In total, the number of RF applications from the endocardium was 128 and that from the epicardium was 109. The mean percentage of the R wave reduction by RF energy was significantly lower in the epicardium than in the endocardium (59% ± 25% vs 69% ± 25%; P = .009). In order to achieve transmural lesions, other approaches such as needle, bipolar, and transcoronary ethanol ablation may be necessary, especially in cases with thickened ventricular wall, as in this HCM patient.17, 18, 19 In this case, we could control VTs clinically with concomitant use of antiarrhythmic drugs. Therefore, combined epicardial and endocardial ablation was useful to modify the substrate for VTs in spite of the residual viable myocardial tissue between the endocardium and epicardium in this case. Conclusion To our knowledge, this is the first case that shows important pathologic information after combined epicardial and endocardial ablation using an open-irrigated catheter in a human heart. This pathologic report showed the limitation of the epicardial RFCA to deliver sufficient RF energy to the myocardium beyond the epicardial adipose tissue, which resulted in residual arrhythmogenic substrate even after combined epicardial and endocardial RFCA.