Immunohistochemical localization of hepatopancreatic phospholipase A ₂ in Hexaplex Trunculus digestive cells

Abstract

Background

Mammalian sPLA ₂-IB localization cell are well characterized. In contrast, much less is known about aquatic primitive ones. The aquatic world contains a wide variety of living species and, hence represents a great potential for discovering new lipolytic enzymes and the mode of digestion of lipid food.

Results

The marine snail digestive phospholipase A ₂ (mSDPLA ₂) has been previously purified from snail hepatopancreas. The specific polyclonal antibodies were prepared and used for immunohistochimical and immunofluorescence analysis in order to determine the cellular location of mSDPLA ₂. Our results showed essentially that mSDPLA ₂ was detected inside in specific vesicles tentatively named (mSDPLA ₂+) granules of the digestive cells. No immunolabelling was observed in secretory zymogene-like cells. This immunocytolocalization indicates that lipid digestion in the snail might occur in specific granules inside the digestive cells.

Conclusion

The cellular location of mSDPLA ₂ suggests that intracellular phospholipids digestion, like other food components digestion of snail diet, occurs in these digestive cells. The hepatopancreas of H. trunculus has been pointed out as the main region for digestion, absorption and storage of lipids.