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Abstract
Radicinin is a phytotoxic and antibiotic metabolite produced by some phytopathogenic
fungi. Precursor administration and cell-free experiments with deoxyradicinin and
radicinin were carried out in Bipolaris coicis H13-3. When deoxyradicinin was administered
to the fungus, radicinin and 3-epi-radicinin formed. When radicinin administered,
3-epi-radicinin was formed. Their formation was confirmed by cell-free experiments.
Deoxyradicinin 3-monooxygenase which catalyzes conversion of deoxyradicinin to radicinin
showed the best activity at 35 °C and pH 7.0, and required NAD+ as co-enzyme. Its
molecular weight was determined to be 130-184 kDa. Radicinin epimerase catalyzing
the reaction of radicinin to 3-epi-radicinin was purified from a cell-free extract.
Radicinin epimerase is a homodimer of a 28 kDa subunit, and its highest activity was
achieved at 30-35 °C and pH 7.0-9.0.