Quantum monitoring of cellular metabolic activities in single mitochondria

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

We demonstrated nanoscale MRI of metabolism in single isolated mitochondria and within living cells using NV centers in diamond.

Abstract

Free radicals play a vital role in all kinds of biological processes including immune responses. However, free radicals have short lifetimes and are highly reactive, making them difficult to measure using current methods. Here, we demonstrate that relaxometry measurement, or T1, inherited from the field of diamond magnetometry can be used to detect free radicals in living cells with subcellular resolution. This quantum sensing technique is based on defects in diamond, which convert a magnetic signal into an optical signal, allowing nanoscale magnetic resonance measurements. We functionalized fluorescent nanodiamonds (FNDs) to target single mitochondria within macrophage cells to detect the metabolic activity. In addition, we performed measurements on single isolated mitochondria. We were able to detect free radicals generated by individual mitochondria in either living cells or isolated mitochondria after stimulation or inhibition.

Related collections

Most cited references 62

Record: found
Abstract: found
Article: not found

Mitochondrial membrane potential.

Ljubava D Zorova, Vasily Popkov, Egor Y Plotnikov … (2018)

The mitochondrial membrane potential (ΔΨm) generated by proton pumps (Complexes I, III and IV) is an essential component in the process of energy storage during oxidative phosphorylation. Together with the proton gradient (ΔpH), ΔΨm forms the transmembrane potential of hydrogen ions which is harnessed to make ATP. The levels of ΔΨm and ATP in the cell are kept relatively stable although there are limited fluctuations of both these factors that can occur reflecting normal physiological activity. However, sustained changes in both factors may be deleterious. A long-lasting drop or rise of ΔΨm vs normal levels may induce unwanted loss of cell viability and be a cause of various pathologies. Among other factors, ΔΨm plays a key role in mitochondrial homeostasis through selective elimination of dysfunctional mitochondria. It is also a driving force for transport of ions (other than H+) and proteins which are necessary for healthy mitochondrial functioning. We propose additional potential mechanisms for which ΔΨm is essential for maintenance of cellular health and viability and provide recommendations how to accurately measure ΔΨm in a cell and discuss potential sources of artifacts.

0 comments Cited 561 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: found

Is Open Access

Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling

Ying Wang, Robyn Branicky, Alycia Noë … (2018)

Wang et al. review the dual role of superoxide dismutases in controlling reactive oxygen species (ROS) damage and regulating ROS signaling across model systems as well as their involvement in human diseases.

0 comments Cited 480 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Identification of ROS using oxidized DCFDA and flow-cytometry.

Evgeniy Eruslanov, Sergei Kusmartsev (2010)

Cells constantly generate reactive oxygen species (ROS) during aerobic metabolism. The ROS generation plays an important protective and functional role in the immune system. The cell is armed with a powerful antioxidant defense system to combat excessive production of ROS. Oxidative stress occurs in cells when the generation of ROS overwhelms the cells' natural antioxidant defenses. ROS and the oxidative damage are thought to play an important role in many human diseases including cancer, atherosclerosis, other neurodegenerative diseases and diabetes. Thus, establishing their precise role requires the ability to measure ROS accurately and the oxidative damage that they cause. There are many methods for measuring free radical production in cells. The most straightforward techniques use cell permeable fluorescent and chemiluminescent probes. 2'-7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is one of the most widely used techniques for directly measuring the redox state of a cell. It has several advantages over other techniques developed. It is very easy to use, extremely sensitive to changes in the redox state of a cell, inexpensive and can be used to follow changes in ROS over time.

0 comments Cited 282 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Sci Adv

Journal ID (iso-abbrev): Sci Adv

Journal ID (publisher-id): SciAdv

Journal ID (hwp): advances

Title: Science Advances

Publisher: American Association for the Advancement of Science

ISSN (Electronic): 2375-2548

Publication date Collection: May 2021

Publication date (Electronic): 19 May 2021

Volume: 7

Issue: 21

Electronic Location Identifier: eabf0573

Affiliations

[1 ]University of Groningen, University Medical Center Groningen, Department of Biomedical Engineering, A. Deusinglaan 1, 9713 AV Groningen, Netherlands.

[2 ]Institute of Physics, École Polytechnique Fédérale de Lausanne (EPFL), CH-1015 Lausanne, Switzerland.

Author notes

[* ]Corresponding author. Email: mayeul.chipaux@ 123456epfl.ch (M.C.); romana.schirhagl@ 123456gmail.com (R.S.)

[†]

These authors contributed equally to this work.

Author information

L. Nie http://orcid.org/0000-0002-9067-3617

A. C. Nusantara http://orcid.org/0000-0002-1654-6089

R. Sharmin http://orcid.org/0000-0003-0230-0165

F. P. Perona Martinez http://orcid.org/0000-0001-8759-9586

M. Chipaux http://orcid.org/0000-0002-2906-6084

R. Schirhagl http://orcid.org/0000-0002-8749-1054

Article

Publisher ID: abf0573

DOI: 10.1126/sciadv.abf0573

PMC ID: 8133708

PubMed ID: 34138746

SO-VID: bcf67767-e93e-4f35-b094-fec09683ff68

Copyright © Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

History

Date received : 30 September 2020

Date accepted : 17 March 2021

Funding

Funded by: doi http://dx.doi.org/10.13039/501100000780, European Commission;

Award ID: 714289

Funded by: CONICYT;

Award ID: 72160222

Funded by: Chinese research counsil;

Award ID: 201706170089

Custom metadata

Copyeditor Samantha Cecilio

Data availability:

Comments

Comment on this article

scite_

Cited by 38

See all cited by

Most referenced authors 611

See all reference authors

- Version 1

Quantum monitoring of cellular metabolic activities in single mitochondria

Read this article at

Abstract

Abstract

Related collections

Nanopublications (single, attributable and machine-readable assertions in scientific literature)

Most cited references 62

Mitochondrial membrane potential.

Superoxide dismutases: Dual roles in controlling ROS damage and regulating ROS signaling

Identification of ROS using oxidized DCFDA and flow-cytometry.

Author and article information

Journal

Affiliations

Author notes

Author information

Article

History

Funding

Categories

Custom metadata

Comments

Comment on this article

Similar content 166

Cited by 38

Most referenced authors 611