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      Attenuated and non‐haemadsorbing (non‐ HAD ) genotype II African swine fever virus ( ASFV ) isolated in Europe, Latvia 2017

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          Abstract

          A non-haemadsorbing (non-HAD) ASF virus (ASFV) genotype II, namely Lv17/WB/Rie1, was isolated from a hunted wild boar in Latvia in 2017. Domestic pigs experimentally infected with the non-HAD ASFV developed a nonspecific or subclinical form of the disease. Two months later, these animals were fully protected when exposed to other domestic pigs infected with a related virulent HAD genotype II ASFV.

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          Genotyping field strains of African swine fever virus by partial p72 gene characterisation.

          J L Edrich, M-L Penrith, James G Thomson … (2003)
          A PCR-based sequencing method was developed which permits detection and characterization of African swine fever virus (ASFV) variants within 5 and 48 h, respectively, of receipt of a clinical specimen. Amplification of a 478 bp fragment corresponding to the C-terminal end of the p72 gene, confirms virus presence with genetic characterization being achieved by nucleotide sequence determination and phylogenetic analysis. The method was applied to 55 viruses including those representative of the major ASF lineages identified previously by restriction fragment length polymorphism (RFLP) analysis. Results confirmed that the p72 genotyping method identifies the same major viral groupings. Characterization of additional viruses of diverse geographical, species and temporal origin using the PCR-based method indicated the presence of ten major ASF genotypes on the African continent, the largest of which comprised a group of genetically homogeneous viruses recovered from outbreaks in Europe, South America, the Caribbean and West Africa (the ESAC-WA genotype). In contrast, viruses from southern and East African countries were heterogeneous, with multiple genotypes being present within individual countries. This study provides a rapid and accurate means of determining the genotype of field and outbreak strains of ASF and is therefore useful for molecular epidemiological clarification of ASF.
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            Molecular Characterization of African Swine Fever Virus, China, 2018

            Shengqiang Ge, Jinming Li, Xiaoxu Fan … (2018)
            On August 3, 2018, an outbreak of African swine fever in pigs was reported in China. We subjected a virus from an African swine fever–positive pig sample to phylogenetic analysis. This analysis showed that the causative strain belonged to the p72 genotype II and CD2v serogroup 8.
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              Genetic Variation among African Swine Fever Genotype II Viruses, Eastern and Central Europe

              Carmina Gallardo, Jovita Fernández-Pinero, Virginia Pelayo … (2014)
              African swine fever virus (ASFV) was first reported in eastern Europe/Eurasia in 2007. Continued spread of ASFV has placed central European countries at risk, and in 2014, ASFV was detected in Lithuania and Poland. Sequencing showed the isolates are identical to a 2013 ASFV from Belarus but differ from ASFV isolated in Georgia in 2007.
              Article 0 comments Cited 100 times – based on 0 reviews     Review now
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                Author and article information

                Journal
                Title: Transboundary and Emerging Diseases
                Abbreviated Title: Transbound Emerg Dis
                Publisher: Wiley
                ISSN (Print): 1865-1674
                ISSN (Electronic): 1865-1682
                Publication date Created: February 27 2019
                Publication date Created: May 2019
                Publication date (Electronic): February 18 2019
                Publication date (Print): May 2019
                Volume: 66
                Issue: 3
                Pages: 1399-1404
                Affiliations
                [1 ]European Union Reference Laboratory for African Swine Fever (EURL), Centro de Investigación en Sanidad Animal INIA‐CISA Madrid Spain
                [2 ]Latvia NRL: Animal Disease Diagnostic Laboratory Institute of Food Safety, Animal Health and Environment “BIOR” Riga Latva
                Article
                DOI: 10.1111/tbed.13132
                PubMed ID: 30667598
                SO-VID: bcf709c6-d4eb-495d-8b23-145dddd198ef
                Copyright © © 2019
                License:

                http://onlinelibrary.wiley.com/termsAndConditions#vor

                http://doi.wiley.com/10.1002/tdm_license_1.1

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