LPP and RYR2 Gene Polymorphisms Correlate with the Risk and the Prognosis of Astrocytoma.

Abstract

Astrocytoma is the most common neuroepithelial tumor. Genetic factors play an important role in the development and prognosis of astrocytoma. So this study focuses on the impact of LPP and RYR2 genes on the occurrence and prognosis of astrocytoma. Rs12594 and rs16835904 in the RYR2 gene and rs1064607, rs3796283, and rs2378456 in the LPP gene were selected and genotyped using Agena MassARRAY in 365 patients and 379 healthy populations. Odds ratios (ORs) and 95% confidence intervals (CIs) were determined using logistic regression to assess the influence of gene polymorphisms on occurrence of astrocytoma. The association between genotype and survival outcomes was performed by the Kaplan-Meier method, the log-rank test, and the Cox regression analysis. The survival rates of patients receiving gross total resection and postoperative chemotherapy were higher than patients receiving near total resection and subtotal resection and without chemotherapy. In recessive model, the patients with LPP rs2378456 CC genotype increased the risk of astrocytoma (OR = 1.43, 95% CI 1.01-2.02, p = 0.042). Stratified analysis shows that RYR2 rs16835904 TC-TT genotype facilitated the risk of astrocytoma in male (OR = 1.93, 95% CI 1.15-3.24, p = 0.011). Cox regression analysis shows that RYR2 rs12594 AA genotype and AG genotype were associated with OS of astrocytoma (AG genotype: HR = 1.62, 95% CI 1.04-2.53, p = 0.034; AA genotype: HR = 1.70, 95% CI 1.08-2.68, p = 0.021). RYR2 and LPP genes were found to affect the occurrence and prognosis of astrocytoma.