Functional indicators of response mechanisms to nitrogen deposition, ozone, and their interaction in two Mediterranean tree species

Abstract

The effects of nitrogen (N) deposition, tropospheric ozone (O ₃) and their interaction were investigated in two Mediterranean tree species, Fraxinus ornus L. (deciduous) and Quercus ilex L. (evergreen), having different leaf habits and resource use strategies. An experiment was conducted under controlled condition to analyse how nitrogen deposition affects the ecophysiological and biochemical traits, and to explore how the nitrogen-induced changes influence the response to O ₃. For both factors we selected realistic exposures (20 kg N ha ^-1 yr ^-1 and 80 ppb h for nitrogen and O ₃, respectively), in order to elucidate the mechanisms implemented by the plants. Nitrogen addition resulted in higher nitrogen concentration at the leaf level in F. ornus, whereas a slight increase was detected in Q. ilex. Nitrogen enhanced the maximum rate of assimilation and ribulose 1,5-bisphosphate regeneration in both species, whereas it influenced the light harvesting complex only in the deciduous F. ornus that was also affected by O ₃ (reduced assimilation rate and accelerated senescence-related processes). Conversely, Q. ilex developed an avoidance mechanism to cope with O ₃, confirming a substantial O ₃ tolerance of this species. Nitrogen seemed to ameliorate the harmful effects of O ₃ in F. ornus: the hypothesized mechanism of action involved the production of nitrogen oxide as the first antioxidant barrier, followed by enzymatic antioxidant response. In Q. ilex, the interaction was not detected on gas exchange and photosystem functionality; however, in this species, nitrogen might stimulate an alternative antioxidant response such as the emission of volatile organic compounds. Antioxidant enzyme activity was lower in plants treated with both O ₃ and nitrogen even though reactive oxygen species production did not differ between the treatments.