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      Life before Stonehenge: The hunter-gatherer occupation and environment of Blick Mead revealed by sedaDNA, pollen and spores

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          Abstract

          The Neolithic and Bronze Age construction and habitation of the Stonehenge Landscape has been extensively explored in previous research. However, little is known about the scale of pre-Neolithic activity and the extent to which the later monumental complex occupied an ‘empty’ landscape. There has been a long-running debate as to whether the monumental archaeology of Stonehenge was created in an uninhabited forested landscape or whether it was constructed in an already partly open area of pre-existing significance to late Mesolithic hunter-gatherers. This is of significance to a global discussion about the relationship between incoming farmers and indigenous hunter-gatherer societies that is highly relevant to both Old and New World archaeology. Here we present the results of plant sedaDNA, palynological and geoarchaeological analysis at the Late hunter-gatherer site complex of Blick Mead at the junction of the drylands of Salisbury Plain and the floodplain of the River Avon, on the edge of the Stonehenge World Heritage Site. The findings are placed within a chronological framework built on OSL, radiocarbon and relative archaeological dating. We show that Blick Mead existed in a clearing in deciduous woodland, exploited by aurochsen, deer and hunter-gatherers for approximately 4000 years. Given its rich archaeology and longevity this strongly supports the arguments of continuity between the Late Mesolithic hunter-gatherers activity and Neolithic monument builders, and more specifically that this was a partially open environment important to both groups. This study also demonstrates that sediments from low-energy floodplains can provide suitable samples for successful environmental assaying using sedaDNA, provided they are supported by secure dating and complementary environmental proxies.

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          obitools: a unix-inspired software package for DNA metabarcoding.

          DNA metabarcoding offers new perspectives in biodiversity research. This recently developed approach to ecosystem study relies heavily on the use of next-generation sequencing (NGS) and thus calls upon the ability to deal with huge sequence data sets. The obitools package satisfies this requirement thanks to a set of programs specifically designed for analysing NGS data in a DNA metabarcoding context. Their capacity to filter and edit sequences while taking into account taxonomic annotation helps to set up tailor-made analysis pipelines for a broad range of DNA metabarcoding applications, including biodiversity surveys or diet analyses. The obitools package is distributed as an open source software available on the following website: http://metabarcoding.org/obitools. A Galaxy wrapper is available on the GenOuest core facility toolshed: http://toolshed.genouest.org.
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            LOSS-ON-IGNITION AS AN ESTIMATE OF ORGANIC MATTER AND ORGANIC CARBON IN NON-CALCAREOUS SOILS

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              From barcodes to genomes: extending the concept of DNA barcoding.

              DNA barcoding has had a major impact on biodiversity science. The elegant simplicity of establishing massive scale databases for a few barcode loci is continuing to change our understanding of species diversity patterns, and continues to enhance human abilities to distinguish among species. Capitalizing on the developments of next generation sequencing technologies and decreasing costs of genome sequencing, there is now the opportunity for the DNA barcoding concept to be extended to new kinds of genomic data. We illustrate the benefits and capacity to do this, and also note the constraints and barriers to overcome before it is truly scalable. We advocate a twin track approach: (i) continuation and acceleration of global efforts to build the DNA barcode reference library of life on earth using standard DNA barcodes and (ii) active development and application of extended DNA barcodes using genome skimming to augment the standard barcoding approach.
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                Author and article information

                Contributors
                Role: Formal analysisRole: InvestigationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: InvestigationRole: VisualizationRole: Writing – review & editing
                Role: ResourcesRole: Writing – review & editing
                Role: Investigation
                Role: Writing – review & editing
                Role: MethodologyRole: ResourcesRole: Writing – review & editing
                Role: Investigation
                Role: Investigation
                Role: ConceptualizationRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: ResourcesRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                27 April 2022
                2022
                : 17
                : 4
                : e0266789
                Affiliations
                [1 ] Department of Geography and Environmental Science, University of Southampton, Southampton, United Kingdom
                [2 ] School of Humanities, The University of Buckingham, Buckingham, United Kingdom
                [3 ] Department of Natural Sciences, Tromsø University Museum, Arctic University of Tromsø, Tromsø, Norway
                [4 ] Department of Geography and Geology, University of Salzburg, Salzburg, Austria
                University at Buffalo - The State University of New York, UNITED STATES
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                https://orcid.org/0000-0001-8914-0998
                https://orcid.org/0000-0002-1604-4898
                Article
                PONE-D-21-38931
                10.1371/journal.pone.0266789
                9045597
                35476794
                c3bbf609-3943-4c99-a45c-a7a4b6222e75
                © 2022 Hudson et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 9 December 2021
                : 25 March 2022
                Page count
                Figures: 5, Tables: 0, Pages: 20
                Funding
                The author(s) received no specific funding for this work. However, the corresponding author did receive funding from the University of Southampton for general fieldwork costs.
                Categories
                Research Article
                Earth Sciences
                Geology
                Geologic Time
                Stone Age
                Mesolithic Period
                Social Sciences
                Archaeology
                Earth Sciences
                Hydrology
                Flooding
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Pollen
                Social Sciences
                Archaeology
                Archaeological Dating
                Biology and Life Sciences
                Paleontology
                Paleopedology
                Earth Sciences
                Paleontology
                Paleopedology
                Earth Sciences
                Soil Science
                Paleopedology
                Earth Sciences
                Geology
                Petrology
                Sediment
                Earth Sciences
                Geology
                Sedimentary Geology
                Sediment
                Earth Sciences
                Geology
                Geologic Time
                Stone Age
                Neolithic Period
                Custom metadata
                All relevant data are within the manuscript and its Supporting Information files.

                Uncategorized
                Uncategorized

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