+1 Recommend
1 collections
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Vaginosis citolítica: presentación de un caso clínico Translated title: Cytolytic vaginosis: presentation of a clinical case


      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          La vaginosis citolítica, descrita hace 30 años como citólisis de Döderlein, es frecuente en mujeres en la edad reproductiva y, por las características de flujo vaginal blanquecino y síntomas clínicos, es indistinguible de la vulvovaginitis micótica. Se presenta el caso de una paciente, con diagnóstico clínico presuntivo de vulvovaginitis por Candida spp. a repetición, tratada empíricamente con antifúngicos por año y medio sin ninguna mejoría. Luego de estudios microbiológicos, la coloración de Gram, demostró la presencia de 50 bacilos grampositivos por campo y abundantes núcleos celulares desnudos. El cultivo resultó puro para Lactobacillus spp., lo que permitió confirmar el diagnóstico de vaginosis citolítica. La paciente fue tratada con ampicilina-sulbactam y no ha vuelto a presentar recidivas. En conclusión, es fundamental determinar el pH vaginal de las pacientes en la consulta, así como practicar una coloración de Gram de la secreción vaginal para poner en evidencia los cambios celulares por el exceso de ácido en la vagina y así evitar tratamientos antifúngicos innecesarios que acrecentarán los trastornos de la microbiota vaginal.

          Translated abstract

          Cytolytic vaginosis, described 30 years ago as Döderlein cytolysis, is common in women of reproductive age and, due to the characteristics of whitish vaginal discharge and clinical symptoms, is indistinguishable from mycotic vulvovaginitis. We describe the case of a patient with presumptive clinical diagnosis of recurrent vulvovaginitis by Candida spp. treated empirically with antifungal agents for one and a half years without improvement. After microbiological studies, Gram staining demonstrated the presence of 50 Gram-positive bacilli per field and abundant nude cell nuclei. The culture recovered pure Lactobacillus spp. which permitted the diagnosis of cytolytic vaginosis. The patient was treated with ampicillin-sulbactam and since, has not had recurrences. In conclusion, it is essential to examine the pH of the patient vaginal discharge, as well as to practice a Gram staining of the vaginal secretion to demonstrate the cellular changes produced by the excess of acid in the vagina and therefore avoid unnecessary antifungal treatments that will produce undue changes of the vaginal microbiota.

          Related collections

          Most cited references10

          • Record: found
          • Abstract: found
          • Article: not found

          Definition and classification of abnormal vaginal flora.

          Studying the vaginal microflora is not only fascinating, with many discoveries to be made, it is also a very practical way to help women get rid of bothersome and sometimes dangerous infections. Gram-stained vaginal preparations, Pap smears, specific cultures, and nucleic acid detection techniques can be used to diagnose the constituents of the vaginal flora, but in trained hands office-based microscopy of a fresh vaginal smear, preferably using a x400 magnification phase-contrast microscope, allows almost every diagnosis and combination of diagnoses imaginable. In this chapter I will address the pros and cons of the tools that are in use to study vaginal flora, and discuss the different types of bacterial flora and the difficulties encountered in reaching the correct diagnosis of pathological conditions. The 'intermediate flora' is addressed separately, and a new entity--'aerobic vaginitis'--is discussed. Future research should focus on the interaction between infecting microorganisms and host defence mechanisms, as both together generate the pathogenicity of these conditions.
            • Record: found
            • Abstract: found
            • Article: not found

            Identification of cytolytic vaginosis versus vulvovaginal candidiasis.

            This study aimed to observe the morphological characteristic of vaginal discharge in patients with cytolytic vaginosis (CV) under the microscope and to identify it in patients with CV and in patients with vulvovaginal candidiasis (VVC).
              • Record: found
              • Abstract: found
              • Article: found
              Is Open Access

              Cytolytic vaginosis: A review

              INTRODUCTION Vaginal discharge is one among the common diseases encountered in women. Trichomoniasis, bacterial vaginosis, and yeast infections are the three most frequent causes of vaginal discharge.[1] In some of the patients who have symptoms and signs of vaginal candidiasis, which is unresponsive to antifungal drugs, a diagnosis of cytolytic vaginosis may have to be suspected.[2] Cytolytic vaginosis is also known as lactobacillus overgrowth syndrome or Doderlein's cytolysis. It is characterized by abundant growth of Lactobacilli resulting in lysis of vaginal epithelial cells; and therefore, it is called as cytolytic vaginosis.[3] LACTOBACILLI IN THE VAGINA Doderlein, first described the normal vaginal flora as, consisting of predominantly of the acid producing gram-positive rods, now referred as lactobacillus species. Healthy women of reproductive age groups are usually colonized by lactobacillus and Gardnerella vaginalis. It is also suggested that the presence of oestrogen and lactobacillus are needed to achieve an optimal vagina pH of 4.0–4.5%. Lactobacilli produce lactic acid from glucose, keeping the vagina at an acidic pH. After puberty under the influence of oestrogen, glycogen is deposited in the vaginal epithelial cells, which is metabolized by vaginal epithelial cells to glucose. Lactobacillus converts glucose to lactic acid.[2 4] Some species of Lactobacilli also produce hydrogen peroxide (H2O2), which is toxic to various microorganisms. This may prevent overgrowth of organisms such as E. coli, Candida species, Gardnerella vaginalis and Mobilincus species. It has also been suggested that H2O2 positive strains of Lactobacilli may also protect against human immunodeficiency virus (HIV) infection. The protective role of various other antimicrobial products such as lactacin B and lactocidin are not clearly established. According to several studies, Lactobacilli builds up a barrier against candidal overgrowth by blocking the adhesion of yeast to vaginal epithelial cells through competition for nutrients.[4] PATHOGENESIS OF CYTOLYTIC VAGINOSIS Normal vaginal flora in adult women within the reproductive age group usually consists of Lactobacilli. Further, Lactobacilli in low numbers (five bacilli per ten squamous cells) in vaginal discharges have been considered as protective factors against vaginal candidiasis.[5] As mentioned earlier, Lactobacilli builds up a barrier against candidal overgrowth by blocking the adhesion of candidal yeast cells to vaginal epithelial cells, through competition for nutrients. Sometimes, a few individuals within the reproductive age group may have overgrowth of Lactobacilli. In these patients, Lactobacilli alone or in combination with other bacteria, may cause damage to the vaginal intermediate epithelium that may result in dissolution of the cells. This dissolution causes dysuria in individuals with cytolytic vaginosis.[6] These individuals are misdiagnosed as candidiasis and do not respond to repeated antifungal therapy regimens for suspected recurrent vulvovaginal candidiasis. Patients who have diabetes mellitus may also develop cytolytic vaginosis as it has been claimed that the Lactobacilli are more abundant in women with high serum glucose levels. It has also been observed that symptoms will be more during luteal phase and it has been suggested that in the luteal phase there is remarkable rise in the number of colonizing Lactobacilli.[3] CLINICAL FEATURES AND DIAGNOSIS Cytolytic vaginosis is characterized by pruritus, dyspareunia, and vulval dysuria. Cyclical increase in symptoms is more pronounced during luteal phase.[6] Cerikeioglu et al. , in their study of 210 women with vaginal discharge and other symptom/signs of genital pathology, suggestive of vulvovaginal candidiasis, observed that fifteen patients (7.1%) were diagnosed with cytolytic vaginosis. All of these cases were in the reproductive age groups of 25–40 years. Five were in the luteal phase, with enhanced complaints of discharge and pruritus.[3] In yet another study, the number of patients diagnosed with cytolytic vaginosis was defined as only five in 101 women with abundant vaginal discharge.[7] In one more study of 271 patients with vulvovaginal complaints, 29 (10.7%) were diagnosed as having suggestive vulvovaginal candidiasis, but only 16 (5.9%) had a confirmed diagnosis of candidiasis.[8] Although not mentioned in the article, remaining cases could have been diagnosed as cytolytic vaginosis, if further investigations were carried out. The signs and symptoms were similar to vulvovaginal candidiasis. While vulvovaginal candidiasis was accepted as an important genital disorder comprising 10-30% of all vulvovaginal pathologies with discharge, cytolytic vaginosis was seen in a proportion of 5-7% in the same patient population and considered as a significant clinical condition.[2] As the signs and symptoms of cytolytic vaginosis mimic vulvovaginal candidiasis, it is important to exclude vulvovaginal candidiasis by investigations. A pH of 4.0-4.5 was detected in patients with cytolytic vaginosis. On Gram's stain, leucocytes were not observed unlike in those with candidiasis. Typical candidial yeast cells were also not found.[2 6] Abundant Lactobacilli covering the fragmented epithelial cells may be confused with the “clue cells” of bacterial vaginosis, these are therefore called as “false clue cells”.[9] Usually there is no confusion between bacterial vaginosis and cytolytic vaginosis. Bacterial vaginosis may be diagnosed by pH measurements and whiff tests. Cytolytic vaginosis patients have an acidic pH of 3.5-4.5 where as in bacterial vaginosis have a pH of more than 4.5. All cases of cytolytic vaginosis will have negative culture results in sabourand dextrose agar (SDA).[3] Following diagnostic criteria have been suggested for cytolytic vaginosis:[6] High risk of suspicion, The absence of Trichomonas, Gardnerella or Candida on wet smear. An increase in number of Lactobacilli. A paucity of white blood cells. Evidence of cytolysis. The presence of discharge. pH between 3.5-4.5. TREATMENT After a correct diagnosis, the treatment is directed towards reducing the number of Lactobacilli by elevating the vaginal pH. Treatment involves douching with sodium bicarbonate solution or using a sodium bicarbonate suppository vaginally. Douches are carried out twice weekly for every two weeks. Solution for douches can be prepared by mixing 1–2 table spoons of baking soda with four cups of warm water. Alternatively, empty gelatin capsules are filled using baking soda and one capsule is inserted intravaginally, twice weekly for every two weeks. These measures help in resolving the symptoms by restoring the normal vaginal environment. If the symptom persists or worsen beyond 2–3 weeks after initiating treatment, re-evaluation is required.[2 6 10] CONCLUSION Thus, any women having an undiagnosed vaginal discharge, the diagnosis of cytolytic vaginosis should be considered as a possible culprit. It is not as common as bacterial vaginosis or candidiasis, but is sometimes confused with them especially with the latter. A misdiagnosis can lead to the patient's suffering and unnecessary medication for other causes of vaginal discharge.

                Author and article information

                Revista de la Sociedad Venezolana de Microbiología
                Rev. Soc. Ven. Microbiol.
                Organo Oficial de la Sociedad Venezolana de Microbiología. (Caracas, DF, Venezuela )
                December 2016
                : 36
                : 2
                : 68-70
                [02] Cumaná Sucre orgnameUniversidad de Oriente orgdiv1Departamento de Biomedicina orgdiv2Laboratorio de Retina del IIBCAUDO
                [03] Carúpano Sucre orgnameHospital Santos Aníbal Dominicci
                [01] orgnameUniversidad de Oriente orgdiv1Laboratorio de Retina del IIBCAUDO
                [04] Ciudad Bolívar orgnameUniversidad de Oriente orgdiv1Escuela de Ciencias de la Salud Dr. Francisco Batistini orgdiv2Departamento de Parasitología y Microbiología Venezuela
                S1315-25562016000200008 S1315-2556(16)03600208


                : 12 October 2016
                : 20 December 2016
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 10, Pages: 3

                SciELO Venezuela

                Casos Clínicos

                cytolytic vaginosis,Lactobacillus spp,coloración de Gram,fracaso terapéutico,vaginosis citolítica,Gram stain,treatment failure


                Comment on this article