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Abstract
Here we develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method
for the determination of digoxin in serum.
The serum samples were extracted with methyl tert-butyl ether using an isotope-labeled
digoxin-d3 as internal standard. The analyte was separated on a reverse phase Capcell
C18 column and detected in positive electrospray ionization multiple reaction monitoring
mass spectrometry.
The chromatographic analysis was carried out within 3 min, but the complete analysis
took longer because of the liquid-liquid extraction. The lower limit of quantification
was 0.1 ng/mL for digoxin. The intra- and inter-batch precisions were less than 12%,
and the bias ranged from -9.1% to 10.7%. The external quality assessment (EQA) results
obtained with the LC-MS/MS method were comparable to target values. Subsequently,
this method has been applied to the therapeutic monitoring of digoxin in a clinical
setting.
In this study, we have developed a rapid and reliable LC-MS/MS method for the therapeutic
monitoring of digoxin in human serum.
(c) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All
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