Risk factors for Cryptosporidium infection in low and middle income countries: A systematic review and meta-analysis

Abstract

Background

Cryptosporidium infection causes gastrointestinal disease and has a worldwide distribution. The highest burden is in developing countries.

Objectives

We sought to conduct a systematic review and meta-analysis to identify Cryptosporidium risk factors in Low and Middle Income countries (LMICs).

Methods

Medline Ovid and Scopus databases were searched with no restriction on year or language of publication. All references were screened independently in duplicate and were included if they presented data on at least 3 risk factors. Meta-analyses using random effects models were used to calculate overall estimates for each exposure.

Results

The most frequently reported risk factors in the 15 included studies were overcrowding, household diarrhoea, poor quality drinking water, animal contact, open defecation/ lack of toilet and breastfeeding. The combined odds ratio for animal contact was 1.98 (95%CI: 1.11–3.54) based on 11 studies and for diarrhoea in the household 1.98 (95%CI: 1.13–3.49) based on 4 studies. Open defecation was associated with a pooled odds ratio of 1.82 (95%CI: 1.19–2.8) based on 5 studies. Poor drinking water quality was not associated with a significant Cryptosporidium risk, odds ratio 1.06 (95%CI: 0.77–1.47). Breastfeeding was protective with pooled odds ratio 0.4 (95%CI: 0.13–1.22), which was not statistically significant.

Conclusions

Based on the included studies, crowded living conditions, animal contact and open defecation are responsible for the majority of Cryptosporidium cases in LMICs. Future studies investigating Cryptosporidium risk factors should have a good study design and duration, include appropriate number of cases, select suitable controls, investigate multiple relevant risk factors, fully report data and perform multivariate analysis.

Author summary

Cryptosporidium is a parasite that causes diarrhoea and is transmitted through faecal contamination of water and food. Though it occurs in developed nations, it is much more prevalent in developing countries and is associated with high mortality in children under 2 years old. In this review, we looked at published studies on factors that increase the risk of contracting cryptosporidiosis in Low and Middle Income Countries. These factors could be targeted to limit the transmission of the disease. Based on the selected studies, the most important risk factors identified were contacts with animals and presence of infected people in the household. Open defecation was also contributing to the risk of infection by this parasite transmitted through the faecal oral route. Breastfeeding was protective from the infection. Poor drinking water was not responsible for causing the disease.