10
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Evaluation of substrate composition and exogenous hormone application on vegetative propagule rooting success of essential oil hemp ( Cannabis sativa L.)

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          To support the rapidly expanding industrial hemp industry, a commercial supply of high-quality starter plants with low genetic variability from nurseries will be key to consistent and efficient cultivation efforts. Rooting success was evaluated across four propagation medias, five rooting hormones, and eight commercially available high-cannabidiol (CBD) essential oil hemp cultivars. Cuttings were placed in a climate-controlled room and assessed for rooting success 12 days after cloning. Rooting success was determined by quantifying total root number, cumulative total root length, and total root mass. Propagation media had the greatest effect on rooting success (13–80%). Rockwool had the highest rooting success resulting in 10-fold increases in rooting traits over the next highest scoring medium (Berger BM6). Hormone applications significantly improved (15- to 18-fold) rooting success compared to no hormone application, while non-statistical differences were observed across auxin hormone concentrations and application methods. Genetic variation in rooting response was observed between cultivars with ‘Cherry Wine’ outperforming all other cultivars with an approximate 20% increase in rooting success over the next highest rooting cultivar, ‘Wife’. Although the ideal combination was not specifically identified in this study, findings provide insight into how rooting hormone application and medium selection impact vegetative propagule rooting success of essential oil hemp.

          Related collections

          Most cited references34

          • Record: found
          • Abstract: not found
          • Article: not found

          New developments in fiber hemp (Cannabis sativa L.) breeding

            Bookmark
            • Record: found
            • Abstract: not found
            • Article: not found

            Key cultivation techniques for hemp in Europe and China

              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              A review of the cultivation and processing of cannabis (Cannabis sativa L.) for production of prescription medicines in the UK.

              The quality demands of the pharmaceutical industry require prescription medicines to be consistent in their active ingredient content. Achieving this, using raw cannabis as a feedstock, is especially challenging. The plant material is extremely inhomogeneous, and the ratios of active ingredients are affected by a range of factors. These include the genetics of the plant, the growing and storage conditions, the state of maturity at harvest, and the methods used to process and formulate the material. The reasons for this variability are described, with particular emphasis on the botanical considerations. To produce the complex botanical medicine Sativex®, which contains the cannabinoids Δ(9)-tetrahydrocannabinol (THC) and cannabidiol (CBD) and a range of other ingredients, GW Pharmaceuticals had to manage these variables. This medicine, for the treatment of spasticity due to multiple sclerosis, is the first cannabis-based medicine to be approved in the UK. The company's methodology for producing this and other chemotypes is described.
                Bookmark

                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole: Project administrationRole: ValidationRole: VisualizationRole: Writing – original draft
                Role: Data curationRole: Formal analysisRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draft
                Role: Funding acquisitionRole: ResourcesRole: SupervisionRole: Writing – review & editing
                Role: ConceptualizationRole: Funding acquisitionRole: InvestigationRole: MethodologyRole: Project administrationRole: ResourcesRole: SupervisionRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                29 July 2021
                2021
                : 16
                : 7
                : e0249160
                Affiliations
                [1 ] Department of Environmental Horticulture, Mid-Florida Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, Apopka, Florida, United States of America
                [2 ] Department of Agronomy, Tropical Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, Homestead, Florida, United States of America
                Beijing Forestry University, CHINA
                Author notes

                Competing Interests: The authors declare that funds were received from Green Roads, LLC and Roseville Farms to conduct this research and fund salaries. This does not alter our adherence to PLOS ONE policies on sharing data and materials.

                [¤]

                Current address: Department of Viticulture and Enology, University of California, Davis, Davis, California, United States of America

                Author information
                https://orcid.org/0000-0002-8512-8091
                https://orcid.org/0000-0001-5153-3083
                Article
                PONE-D-21-08119
                10.1371/journal.pone.0249160
                8320905
                34324510
                d2c9adeb-7492-4dc1-b1ba-9b76bacc4d05
                © 2021 Campbell et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 12 March 2021
                : 2 July 2021
                Page count
                Figures: 3, Tables: 0, Pages: 12
                Funding
                Funded by: Green Roads, LLC
                Award Recipient :
                Funded by: Roseville Farms
                Award Recipient :
                Funded by: ANO HEMP LLC
                Award Recipient :
                Funded by: Green Point Research
                Award Recipient :
                Funded by: UF/IFAS Office of the Dean and Research
                Award Recipient :
                The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. This project was made possible by financial support from Green Roads, LLC and the UF/IFAS Office of the Dean and Research. Green Point Research, Green Roads LLC, and ANO Colorado LLC donated the cultivars used in this research. Steven Anderson was funded by Roseville Farm’s UF/IFAS Florida Industrial Hemp Endowment contribution.
                Categories
                Research Article
                Biology and Life Sciences
                Agriculture
                Agronomy
                Plant Products
                Hemp
                Biology and Life Sciences
                Agriculture
                Crop Science
                Plant Products
                Hemp
                Biology and Life Sciences
                Biochemistry
                Hormones
                Plant Hormones
                Auxins
                Biology and Life Sciences
                Biochemistry
                Plant Biochemistry
                Plant Hormones
                Auxins
                Biology and Life Sciences
                Plant Science
                Plant Biochemistry
                Plant Hormones
                Auxins
                Biology and Life Sciences
                Agriculture
                Agronomy
                Plant Products
                Essential Oils
                Biology and Life Sciences
                Agriculture
                Crop Science
                Plant Products
                Essential Oils
                Medicine and Health Sciences
                Pharmacology
                Behavioral Pharmacology
                Recreational Drug Use
                Cannabis
                Biology and Life Sciences
                Biochemistry
                Hormones
                Biology and Life Sciences
                Plant Science
                Plant Anatomy
                Leaves
                Physical Sciences
                Materials Science
                Material Properties
                Porosity
                Medicine and Health Sciences
                Pharmaceutics
                Drug Therapy
                Hormonal Therapy
                Custom metadata
                All relevant data are within the manuscript and its S1 File.

                Uncategorized
                Uncategorized

                Comments

                Comment on this article