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      Ocorrência de bactérias diazotróficas associadas a cultivares de sorgo forrageiro Translated title: Occurrence of diazotrophic bacteria associated with forage sorghum cultivars

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          Abstract

          O presente estudo objetivou avaliar a ocorrência de bactérias diazotróficas associadas ao sorgo, selecionar cultivares eficientes na associação com bactérias diazotróficas e identificar os isolados mais eficientes em fixar nitrogênio atmosférico (N²) e produzir ácido indol-acético (AIA). Utilizaram-se 14 cultivares de sorgo forrageiro em vasos, com dois níveis de N: 0 e 130kg ha-1 de N. A seleção de cultivares foi baseada na associação com bactérias diazotróficas e na eficiência de absorção de nitrogênio das cultivares. Para o isolamento das bactérias, foram utilizados diferentes meios de enriquecimento semi-sólido e, após o isolamento, foram quantificadas a produção de AIA e a quantidade de N² fixada pelos isolados. A ocorrência de bactérias diazotróficas foi constatada em todas as cultivares avaliados sendo que a distribuição das bactérias isoladas foi influenciada pelo genótipo da planta. Todos os isolados foram aptos em fixar N² e produzir AIA in vitro.

          Translated abstract

          The present study aimed to evaluate the occurrence of diazotrophic bacterial associated with sorghum plants, to select efficient sorghum cultivars associated with diazotrophic bacterial, as well as to identify isolates efficient on nitrogen fixation (N²) and indole acetic acid (IAA) production. Fourteen forage sorghum cultivars were cropped in jars under two levels of nitrogen fertilization: 0 and 130kg ha-1 of N. The selection of sorghum cultivars was based on the association with diazothophic bacterial and on the efficiency of nitrogen absorption. Semi-solid enrichment medium was used for the isolation of the diazotrophic bacterial. The occurrence of diazotrophics was verified in all cultivars of sorghum evaluated and, the distribution of the isolates was affected by the plant genotype. All the isolates were able to fix N² and to produce IAA in vitro.

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          Most cited references17

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          A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding

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            Sistema Brasileiro de Classificação do solo

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              Azospirillum-plant relationships: physiological, molecular, agricultural, and environmental advances (1997-2003).

              This review presents a critical and comprehensive documentation and analysis of the developments in agricultural, environmental, molecular, and physiological studies related to Azospirillum cells, and to Azospirillum interactions with plants, based solely on information published between 1997 and 2003. It was designed as an update of previous reviews (Bashan and Levanony 1990; Bashan and Holguin 1997a), with a similar scope of interest. Apart from an update and critical analysis of the current knowledge, this review focuses on the central issues of Azospirillum research today, such as, (i) physiological and molecular studies as a general model for rhizosphere bacteria; (ii) co-inoculation with other microorganisms; (iii) hormonal studies and re-consideration of the nitrogen contribution by the bacteria under specific environmental conditions; (iv) proposed Azospirillum as a non-specific plant-growth-promoting bacterium; (v) re-introduction of the "Additive Hypothesis," which suggests involvement of multiple mechanisms employed by the bacteria to affect plant growth; (vi) comment on the less researched areas, such as inoculant and pesticide research; and (vii) proposes possible avenues for the exploitation of this bacterium in environmental areas other than agriculture.
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                Author and article information

                Journal
                cr
                Ciência Rural
                Cienc. Rural
                Universidade Federal de Santa Maria (Santa Maria, RS, Brazil )
                0103-8478
                1678-4596
                June 2007
                : 37
                : 3
                : 727-733
                Affiliations
                [01] Porto Alegre RS orgnameUniversidade Federal do Rio Grande do Sul orgdiv1Departamento de Ciência do Solo Brasil fcamargo@ 123456ufrgs.br
                Article
                S0103-84782007000300019 S0103-8478(07)03700319
                10.1590/S0103-84782007000300019
                dde092a2-85fd-4ebf-a0c1-e913fe650d66

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 22 March 2006
                : 25 October 2006
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 16, Pages: 7
                Product

                SciELO Brazil

                Categories
                Ciência do Solo

                bactérias fixadoras de nitrogênio,produção de ácido indol-acético,nitrogen fixing bacteria,indole acetic acid production

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