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An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry.

Author(s): A Pillez, G Tramu, J Leonardelli

Publication date: 1978-03-31

Journal: Journal of Histochemistry and Cytochemistry

Keywords: Adrenocorticotropic Hormone, analysis, Animals, Antibodies, Guinea Pigs, Immunoenzyme Techniques, Immunologic Techniques, Luteinizing Hormone, Pituitary Gland

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Abstract

The method described in this paper is available for removal of antibodies retained by tissue antigen after immunohistochemical staining. Its application to the antehypophysis has allowed the successive or the simultaneous localization of two different hormones.

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PubMed ID:: 207771

DOI:: 10.1177/26.4.207771

ScienceOpen disciplines: Chemistry

Keywords: Adrenocorticotropic Hormone,analysis,Animals,Antibodies,Guinea Pigs,Immunoenzyme Techniques,Immunologic Techniques,Luteinizing Hormone,Pituitary Gland

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ScienceOpen disciplines: Chemistry

Keywords: Adrenocorticotropic Hormone, analysis, Animals, Antibodies, Guinea Pigs, Immunoenzyme Techniques, Immunologic Techniques, Luteinizing Hormone, Pituitary Gland

An efficient method of antibody elution for the successive or simultaneous localization of two antigens by immunocytochemistry.

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