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      Estereología Comparativa entre las Glándulas del Complejo Prostático del Conejo Oryctolagus cunniculus Translated title: Comparative Stereology between the Glands of the Prostatic Complex in the Rabbit Oryctolagus cunniculus

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          Abstract

          El objetivo del estudio fue determinar algunos parámetros estereológicos de las glándulas que conforman el complejo prostático en el conejo y así, sentar las bases para estudios morfofuncionales. Se utilizaron 5 conejos (Oryctolagus cuniculus), machos, adultos y clínicamente sanos, mantenidos en el Bioterio de la Facultad de Medicina de la Universidad de La Frontera, Temuco, Chile. Fue disecada la región pélvica y se aisló el complejo prostático de las otras glándulas anexas. Las muestras fueron fijadas en Bouin, durante 24 horas y procesadas para su inclusión en paraplast. Se realizaron cortes seriados de 5 |Jm de espesor, los cuales fueron teñidos con hematoxilina eosina, para su análisis estéreo lógico. Los resultados mostraron que el complejo prostático del conejo está formado por 4 glándulas: próstata (craneal a la glándula vesicular), propróstata (caudal a la glándula vesicular y craneal a la próstata) y dos parapróstata (inferiores y lateralmente situadas a la propróstata). El promedio de células glandulares en la próstata fue de 7,3 x 10(6)/ mm³ (D. E. 1,8 x 10(6)); en la propróstata fue de 10,33 x 10(6)/ mm³ (D. E. 2,45 x 10(6)); en la parapróstata derecha fue de 13,57 x 10(6)/ mm³ (D. E. 2,84 x 10(6)) y en la parapróstata izquierda fue de 11,91 x 10(6)/mm³ (D. E. 2,97 x 10(6)).E1 porcentaje promedio de células glandulares en la próstata fue de 14,12% (D. E. 3,01); en la propróstata fue de 13,56% (D. E. 3,13); en la parapróstata derecha fue de 18,80% (D. E. 3,04) y en la parapróstata izquierda de 18,59% (D. E. 3,43). Las glándulas del complejo prostático presentan diferencias morfológicas entre sí y, en especial, en sus aspectos estereológicos, siendo las parapróstatas las que presentaron un mayor número de células glandulares y mayor porcentaje de tejido glandular (semejante a la glándula bulbouretral), lo que podría indicar que cada una de las glándulas de este complejo cumple roles funcionales específicos en los procesos reproductivos.

          Translated abstract

          The aim of this study was to determine some stereological parameters of the glands of the prostatic complex in the rabbit and thereby setting the basis for future morphofunctional studies. Five rabbits (Oryctolagus cuniculus), males, adults, clinically healthy and mantained in the Biotery of the Faculty of Medicine of the Universidad de La Frontera, Temuco, Chile, were used. The pelvic region was dissected and the prostatic complex of the other glands was isolated. The samples were fixed in Bouin solution for 24 hours and processed for inclusion in paraplast. Serial cuts of 5mm which were stained with hematoxilin eosin for stereological analysis. The results showed that the prostatic complex of the rabbit was composed of four glands: prostate (cranial to vesicular gland), proprostate (caudal to vesicular gland and cranial to the prostate) and two paraprostates (inferior and lateral to the prostate). The average of the glandular cells in the prostate was 7,3 x 107 mm³ (S. D. 1,8 x 10s); in the proprostate was 10,33 x 107 mm³ (S. D. 2,45 x 10s); in the right paraprostate was 13,57 x 107 mm³ (S. D. 2,84 x 10s) and in the left paraprostate was 11,91 x 107 mm³ (S. D. 2,97 x 10s). The percentage average of the glandular cells in the prostate was 14,12% (S. D. 3,01); in the proprostate was 13,56% (S. D. 3,13); in the right paraprostate was 18,80% (S. D. 3,04) and in the left paraprostate was 18,59% (S. D. 3,43). The gland of the prostatic complex show morphological differences among themselves and in particular, its stereological aspects, with paraprostates which showed an increased number of glandular cells and higher percentage of glandular tissue ( similar to the bulbourethral gland), which could indicate that each of the glands of this complex plays specific functional roles in the reproductives processes.

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          Most cited references15

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          A simple method for volumetry of organs in quantitative stereology.

          W Scherle (1970)
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            Stereological methods: practical methods of biological morphometry

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              The anatomy of the reproductive system in male Dutch rabbits (Oryctolagus cuniculus) with special emphasis on the accessory sex glands.

              The morphology and microscopic anatomy of the reproductive organs and colliculus seminalis of the male rabbit are described and illustrated. Special attention is given to the accessory sex glands, because not all have been accurately identified previously. The terminology suggested for the accessory glands (with other terms commonly used in the literature in parentheses) follows: glandula vesicularis (glandula seminalis, vesicular seminalis), proprostata (glandula vesicularis, coagulating gland, prostata), prostata, paraprostata (glandula Cowperi superior), glandula bulbourethralis (glandula Cowperi inferior). The English equivalents are vesicular, proprostate, prostate, paraprostate and bulbourethral glands. Organs were obtained from 39 adult Dutch-belted rabbits that averaged 2,023 g in bodyweight. Organ weights (means and standard deviations) were as follows: testis, 2.035 +/- 0.529 g; caput epididymidis, 0.264 +/- 0.087 g; corpus epididymidis, 0.046 +/- 0.019 g; cauda epididymidis, 0.398 +/- 0.123 g; proximal part of the ductus deferens, 0.098 +/- 0.026 g; ampulla of the ductus deferens, 0.177 +/- 0.069 g; vesicular gland, 0.529 +/- 1.169 g; proprostate gland, 0.633 +/- 0.304 g; prostate gland, 0.411 +/- 0.181 g; paraprostate gland, 0.040 +/- 0.019 g; bulbourethral gland, 0.390 +/- 0.133 g; and epididymal fat pad, 0.545 +/- 0.339 g. Correlations among organ weights were calculated and interrelationships among them and bodyweight are discussed.
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                Author and article information

                Journal
                ijmorphol
                International Journal of Morphology
                Int. J. Morphol.
                Sociedad Chilena de Anatomía (Temuco, , Chile )
                0717-9502
                March 2009
                : 27
                : 1
                : 205-210
                Affiliations
                [02] Temuco orgnameUniversidad de La Frontera Chile
                [01] Temuco orgnameUniversidad Autónoma de Chile Chile
                Article
                S0717-95022009000100035 S0717-9502(09)02700135
                10.4067/S0717-95022009000100035
                e0433452-ce08-498f-9efa-007403a41c4d

                This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

                History
                : 22 December 2008
                : 15 September 2008
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 16, Pages: 6
                Product

                SciELO Chile


                Glándulas,Anatomy,Stereology,Rabbit,Prostatic complex,Glands,Anatomía,Estereología,Conejo,Complejo prostático

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