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      Stable isotope analysis of human bone from Ganj Dareh, Iran, ca. 10,100 calBP

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          Abstract

          We report here on stable carbon, nitrogen, and sulfur isotope values from bone collagen of human (n = 20) and faunal (n = 11) remains from the Early Neolithic site of Ganj Dareh, Iran, dating to ca. 10,100 cal. BP. Our focus explores how isotope values of human bone vary by age and sex, and evaluates dietary practices at this site. It also provides a baseline for future studies of subsistence in the early Holocene Central Zagros Mountains, from the site with the first evidence for human ovicaprid management in the Near East. Human remains include individuals of all age groups for dietary reconstruction, as well two Ottoman intrusive burials for temporal and cultural comparison. All analyzed individuals exhibited δ 13C and δ 15N values consistent with a diet based heavily on C 3 terrestrial sources. There is no statistically significant difference between the isotopic compositions of the two sexes, though males appear to show larger variations compared to females. Interesting patterns in the isotopic compositions of the subadults suggested weaning children may be fed with supplements with distinctive δ 13C values. Significant difference in sulfur isotope values between humans and fauna could be the earliest evidence of transhumance and could identify one older adult male as a possible transhumant shepherd. Both Ottoman individuals had distinctively different δ 13C, δ 15N, and δ 34S values compared to the Neolithic individuals. This is the first large scale analysis of human stable isotopes from the eastern end of the early Holocene Fertile Crescent. It provides a baseline for future intersite exploration of stable isotopes and insight into the lifeways, health, and processes of neolithisation associated with the origins of goat domestication at Ganj Dareh and the surrounding Central Zagros.

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          Preparation and characterization of bone and tooth collagen for isotopic analysis

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            New method of collagen extraction for radiocarbon dating.

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              Genomic insights into the origin of farming in the ancient Near East

              We report genome-wide ancient DNA from 44 ancient Near Easterners ranging in time between ~12,000-1,400 BCE, from Natufian hunter-gatherers to Bronze Age farmers. We show that the earliest populations of the Near East derived around half their ancestry from a ‘Basal Eurasian’ lineage that had little if any Neanderthal admixture and that separated from other non-African lineages prior to their separation from each other. The first farmers of the southern Levant (Israel and Jordan) and Zagros Mountains (Iran) were strongly genetically differentiated, and each descended from local hunter-gatherers. By the time of the Bronze Age, these two populations and Anatolian-related farmers had mixed with each other and with the hunter-gatherers of Europe to drastically reduce genetic differentiation. The impact of the Near Eastern farmers extended beyond the Near East: farmers related to those of Anatolia spread westward into Europe; farmers related to those of the Levant spread southward into East Africa; farmers related to those from Iran spread northward into the Eurasian steppe; and people related to both the early farmers of Iran and to the pastoralists of the Eurasian steppe spread eastward into South Asia.
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                Author and article information

                Contributors
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: ResourcesRole: SupervisionRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: ConceptualizationRole: Formal analysisRole: InvestigationRole: MethodologyRole: Project administrationRole: SoftwareRole: ValidationRole: VisualizationRole: Writing – original draftRole: Writing – review & editing
                Role: ValidationRole: Writing – original draftRole: Writing – review & editing
                Role: ConceptualizationRole: Data curationRole: Formal analysisRole: Funding acquisitionRole: MethodologyRole: Project administrationRole: ResourcesRole: SoftwareRole: SupervisionRole: ValidationRole: Writing – review & editing
                Role: Editor
                Journal
                PLoS One
                PLoS One
                plos
                plosone
                PLoS ONE
                Public Library of Science (San Francisco, CA USA )
                1932-6203
                2 March 2021
                2021
                : 16
                : 3
                : e0247569
                Affiliations
                [1 ] Department of Archaeology, Simon Fraser University, Burnaby, BC, Canada
                [2 ] EA–Eco-anthropologie (UMR 7206), Muséum National d’Histoire Naturelle, CNRS, Université Paris Diderot, Paris, France
                [3 ] UMR 7269, LAMPEA, Aix-Marseille Université, CNRS, Minist Culture, Aix-en-Provence, France
                [4 ] Department of Anthropology, University of Winnipeg, Winnipeg, MB, Canada
                Museo delle Civiltà, ITALY
                Author notes

                Competing Interests: The authors have declared that no competing interests exist.

                Author information
                https://orcid.org/0000-0001-9539-7271
                https://orcid.org/0000-0002-4100-5524
                Article
                PONE-D-20-29910
                10.1371/journal.pone.0247569
                7924805
                33651827
                e4b017af-856f-4093-ac14-14e4b8ae6030
                © 2021 Merrett et al

                This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                History
                : 22 September 2020
                : 9 February 2021
                Page count
                Figures: 4, Tables: 3, Pages: 15
                Funding
                Funded by: funder-id http://dx.doi.org/10.13039/501100000155, Social Sciences and Humanities Research Council of Canada;
                Award ID: 950-231396
                Award Recipient :
                Funded by: funder-id http://dx.doi.org/10.13039/501100002790, Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada;
                Award ID: 03836-2015
                Award Recipient :
                • (1) SSHRC:: • MPR • 950-231396 • Social Sciences and Humanities Research Council of Canada • https://www.sshrc-crsh.gc.ca The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. • (2) NSERC MPR • 03836-2015 Natural Sciences and Engineering Research Council of Canada https://www.nserc-crsng.gc.ca The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
                Categories
                Research Article
                Research and Analysis Methods
                Chemical Characterization
                Isotope Analysis
                Earth Sciences
                Geology
                Geologic Time
                Stone Age
                Neolithic Period
                Physical Sciences
                Chemistry
                Chemical Elements
                Sulfur
                Biology and Life Sciences
                Nutrition
                Diet
                Medicine and Health Sciences
                Nutrition
                Diet
                Biology and Life Sciences
                Anatomy
                Osteology
                Medicine and Health Sciences
                Anatomy
                Osteology
                Biology and Life Sciences
                Biochemistry
                Proteins
                Collagens
                Physical Sciences
                Physics
                Particle Physics
                Composite Particles
                Atoms
                Isotopes
                Physical Sciences
                Physics
                Particle Physics
                Composite Particles
                Atoms
                Isotopes
                Stable Isotopes
                Custom metadata
                All relevant data are within the manuscript.

                Uncategorized
                Uncategorized

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