Trehalose improves survival of electrotransfected mammalian cells.

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Abstract

Electropermeabilization is widely used for introduction of DNA and other foreign molecules into eukaryotic cells. However, conditions yielding the greatest molecule uptake and gene expression can result in low cell survival. In this study, we assessed the efficiency of trehalose for enhancing cell viability after excessive electropermeabilization. This disaccharide was chosen because of its capability of stabilizing cell membranes under various stressful conditions, such as dehydration and freezing.

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Journal

Journal ID (iso-abbrev): Cytometry

Title: Cytometry

Publisher: Wiley

ISSN: 0196-4763

ISSN (Print): 0196-4763

Publication date (Electronic): Nov 01 2001

Volume: 45

Issue: 3

Affiliations

[1 ] Lehrstuhl für Biotechnologie, Biozentrum, Universität Würzburg, Am Hubland, Würzburg, Germany.

Article

Publisher Item ID: 10.1002/1097-0320(20011101)45:3<161::AID-CYTO1159>3.0.CO;2-7

DOI: 10.1002/1097-0320(20011101)45:3<161::aid-cyto1159>3.0.co;2-7

PubMed ID: 11746084

SO-VID: e61b154d-7c40-404e-9342-f1162d4d1ad3

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