Generation of orthotopic patient-derived xenograft models for pancreatic cancer using tumor slices

Summary

Orthotopic patient-derived xenograft models recapitulate the genomic complexity of the original tumor and some aspects of local microenvironment, useful for rapid development and validation of personalized treatment strategies. Here, we precisely describe a protocol for generating tumor slices from human or murine-derived pancreatic cancer. They are then implanted directly into the murine pancreas, monitored using ultrasound, with a 90% success rate. This assay creates a clinically relevant in vivo model facilitating personalized treatment development.

Graphical abstract

Highlights

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  A highly efficient protocol for patient-derived xenograft (PDX) mouse model generation

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  Tumor slice preparation from human or murine-derived pancreatic tumor

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  Direct orthotopic implantations of tumor slices into mouse pancreas

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  Tumor slices, compared to cell lines or fragments, improves engraftment to about 90%

Abstract

Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Abstract

Orthotopic patient-derived xenograft models recapitulate the genomic complexity of the original tumor and some aspects of local microenvironment, useful for rapid development and validation of personalized treatment strategies. Here, we precisely describe a protocol for generating tumor slices from human or murine-derived pancreatic cancer. They are then implanted directly into the murine pancreas, monitored using ultrasound, with a 90% success rate. This assay creates a clinically relevant in vivo model facilitating personalized treatment development.