Enhancement of Ammonium Oxidation at Microoxic Bioanodes

Abstract

Bioelectrochemical systems (BESs) are considered to be energy-efficient to convert ammonium, which is present in wastewater. The application of BESs as a technology to treat wastewater on an industrial scale is hindered by the slow removal rate and lack of understanding of the underlying ammonium conversion pathways. This study shows ammonium oxidation rates up to 228 ± 0.4 g-N m ^–3 d ^–1 under microoxic conditions (dissolved oxygen at 0.02–0.2 mg-O ₂/L), which is a significant improvement compared to anoxic conditions (120 ± 21 g-N m ^–3 d ^–1). We found that this enhancement was related to the formation of hydroxylamine (NH ₂OH), which is rate limiting in ammonium oxidation by ammonia-oxidizing microorganisms. NH ₂OH was intermediate in both the absence and presence of oxygen. The dominant end-product of ammonium oxidation was dinitrogen gas, with about 75% conversion efficiency in the presence of a microoxic level of dissolved oxygen and 100% conversion efficiency in the absence of oxygen. This work elucidates the dominant pathways under microoxic and anoxic conditions which is a step toward the application of BESs for ammonium removal in wastewater treatment.

Abstract

We achieved high ammonium oxidation rates at bioanodes by introducing low levels of oxygen and revealed the rate limiting step and the pathways of ammonium oxidation at bioanodes.