The knowledge about the distribution of Ephemeroptera in Brazil has increased in recent years, contributing to reduce the gaps about its distribution in this country. This paper presents an updated checklist of the species of Ephemeroptera from Pernambuco state, Northeastern Brazil based on bibliography and collections from numerous rivers and streams of 27 counties and 74 localities. In this study, 18 species and 11 genera are recorded for the first time from Pernambuco state, increasing from 43 to 61 the number of known species. With these results, Pernambuco becomes the state with highest number of species in Northeastern Region of Brazil. Moreover, Caenis chamie is reported for the first time from Brazil.