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      Morfometría de larvas de Oestrus ovis (Diptera: Oestridae) de ovinos, en Cuba Translated title: Morphometric characterization of Oestrus ovis (Diptera: Oestridae) larvae from infested sheep in Cuba

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          Abstract

          Con el objetivo de caracterizar morfométricamente los estadios parasitarios de Oestrus ovis, se colectaron 100 larvas de cada uno de los diferentes estadios (L1, L2, L3) de hospederos infestados necropsiados, colocadas en etanol 70° y medidas bajo un microscopio estéreo. Los resultados obtenidos fueron comparados con los de otros autores. Se realizó un estudio de los ejemplares con vistas a la descripción de características morfológicas que permitan criterios diagnósticos. Se tomaron microfotografías de diferentes regiones de los estadios larvarios.

          Translated abstract

          In order to characterize the parasitic stages of Oestrus ovis morphometrically, 100 larvae from each stage (L1, L2, L3) were collected from necrotic infested hosts, placed in ethanol 70° and measured under a stereo microscope. The results were compared with those of other authors. The specimens were studied with a view to the description of morphological features that allow diagnostic criteria. Micrographs were taken from different regions of the larval stages.

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          Parasitología y enfermedades parasitarias de animales domésticos

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            Sheep oestrosis (Oestrus ovis Linné 1761, Diptera: Oestridae) in Sardinia, Italy.

            Oestrosis, the nasal myiasis of sheep and goats, is caused by the larvae of Oestrus ovis L. 1758 (Diptera, Oestridae) that develop from the first to the third stage larva in the nasal cavities and frontal sinuses of affected animals. The authors report the results of an epidemiological study of oestrosis of sheep in Sardinia, Italy. Heads of 6-month to 10-year-old Sardinian sheep (n=566) from 124 free-ranging flocks were examined for the presence and location O. ovis larvae from December 1996 to November 1997. Larvae were collected, counted, and larval stages were identified. O. ovis larvae were found in 100% of examined flocks and in 91% (514/566) of examined sheep. The monthly prevalence ranged from 69% in May to 100% in July. First stage larvae were found in 82% (463) of all heads examined, second stage larvae in 65% (367) and third stage larvae in 10% (56). The majority of sheep harboured first stage larvae, with prevalences of over 80% throughout most of the study period. The prevalence of O. ovis found in this study of Sardinian sheep is the highest reported in the Mediterranean area. The high percentage of first stage larvae found throughout the entire study period may be due to a brief period of decreased rate of larval maturation, in particular in December 1996 (96%) and January-October 1997 (94%). Third stage larvae were consistently present, often however, with extremely low prevalences compared to total larval burden.
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              Proteolytic activity in salivary gland products of sheep bot fly (Oestrus ovis) larvae.

              This study identified and characterized hydrolytic enzymes in salivary gland products of Oestrus ovis larvae. Third instars were collected from the heads of slaughtered goats. Salivary glands were extracted, their products obtained by centrifugation and the enzymatic profile determined. Optimum pH, temperature of maximum proteolytic activity, thermal stability, and resistance of salivary gland products were determined on collagen and subclasses of proteases were identified using protease inhibitors. Zymograms were used to determine the molecular weight of proteases. Antigenic protein bands were revealed by immunoblotting using sera obtained from experimentally infested goats. Seven positive enzymatic activities were detected in salivary gland products: acid phosphatase, naphthol-AS-BI-phosphohydrolase, esterase (C4), esterase lipase (C8), leucine arylamidase, alpha-glucosidase and N-acetyl-beta-glucosaminidase. Optimum pH for proteolytic activity was 8.0; proteolytic activity increased with temperature (10-50 degrees C) then drastically decreased at 60 degrees C. Proteases in O. ovis salivary gland products belong to the serine subclass. In Zymograms, bands of proteolytic activity were detected in the 20-63 kDa range; the immunoblot showed three antigenic bands, one of them related to a protease band (63 kDa). Serine proteases in O. ovis salivary gland products are most likely involved in larval nutrition and host immuno-modulation.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rsa
                Revista de Salud Animal
                Rev Salud Anim.
                Centro Nacional de Sanidad Agropecuaria (La Habana )
                2224-4700
                December 2012
                : 34
                : 3
                : 184-187
                Affiliations
                [1 ] Centro de Desarrollo de la Montaña (CDM) Cuba
                [2 ] Centro Nacional de Sanidad Agropecuaria (CENSA) Cuba
                [3 ] Departamento de Producción Agrícola y Animal México
                Article
                S0253-570X2012000300008
                ec07142a-fe0e-4a82-86f6-19e3e4a9ad55

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Cuba

                Self URI (journal page): http://scielo.sld.cu/scielo.php?script=sci_serial&pid=0253-570X&lng=en
                Categories
                ACOUSTICS

                Acoustics
                Oestrus ovis,morphometric,sheep,oestrosis,morfometría,ovinos
                Acoustics
                Oestrus ovis, morphometric, sheep, oestrosis, morfometría, ovinos

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