To develop and test in animal studies ex vivo and in vivo, an intravascular (IV) MRI-guided high-intensity focused ultrasound (HIFU) ablation method for targeting perivascular pathology with minimal injury to the vessel wall.
IV-MRI antennas were combined with 2–4mm diameter water-cooled IV-ultrasound ablation catheters for IV-MRI on a 3T clinical MRI scanner. A software interface was developed for monitoring thermal dose with real-time MRI thermometry, and an MRI-guided ablation protocol developed by repeat testing on muscle and liver tissue ex vivo. MRI thermal dose was measured as cumulative equivalent minutes at 43°C (CEM 43). The IV-MRI IV-HIFU protocol was then tested by targeting perivascular ablations from the inferior vena cava of two pigs in vivo. Thermal dose and lesions were compared by gross and histological examination.
Ex-vivo experiments yielded a 6-minute ablation protocol with the IV-ultrasound catheter coolant at 3–4°C, a 30ml/min flow rate, and 7W ablation power. In 8 experiments, 5–10mm thick thermal lesions of area 0.5–2cm 2 were produced that spared 1–2mm margins of tissue abutting the catheters. The radial depths, areas and preserved margins of ablation lesions measured from gross histology were highly correlated (r≥0.79) with those measured from the CEM 43=340 necrosis threshold determined by MRI thermometry. The psoas muscle was successfully targeted in the two live pigs, with the resulting ablations controlled under IV-MRI guidance.