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      Pollen morphology of Brazilian species of Verbesina L. (Heliantheae - Asteraceae)

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          Abstract

          Abstract There are nine species of the plant genus Verbesina in Brazil, which are distributed in the Northeast, Center-West, Southeast and South regions of the country. The objective of the present study was to describe the pollinic morphology of eight of these species to better characterize them and evaluate potential species-level taxonomic characters. Acetolysed pollen material was measured within seven days under light microscopy, while non-acetolyzsed pollen grains were used for scanning electronic microscopy. The pollen grains were found to be oblate-spheroidal, medium-sized, isopolar, monads that are 3-colporate with a subtriangular amb, a small polar area, a long colpus, a lalongate endoaperture, a caveate exine and an echinate sexine. Although the shape of the pollen grains of these Brazilian species of Verbesina is homogeneous, some attributes were observed to be useful for characterizing the species, such as exine thickness, distance between spines and side of the apocolpus.

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          Macroevolutionary dynamics in the early diversification of Asteraceae.

          Spatial and temporal differences in ecological opportunity can result in disparity of net species diversification rates and consequently uneven distribution of taxon richness across the tree of life. The largest eudicotyledonous plant family Asteraceae has a global distribution and at least 460 times more species than its South American endemic sister family Calyceraceae. In this study, diversification rate dynamics across Asteraceae are examined in light of the several hypothesized causes for the family's evolutionary success that could be responsible for rate change. The innovations of racemose capitulum and pappus, and a whole genome duplication event occurred near the origin of the family, yet we found the basal lineages of Asteraceae that evolved in South America share background diversification rates with Calyceraceae and their Australasian sister Goodeniaceae. Instead we found diversification rates increased gradually from the origin of Asteraceae approximately 69.5Ma in the late Cretaceous through the Early Eocene Climatic Optimum at least. In contrast to earlier studies, significant rate shifts were not strongly correlated with intercontinental dispersals or polyploidization. The difference is due primarily to sampling more backbone nodes, as well as calibrations placed internally in Asteraceae that resulted in earlier divergence times than those found in most previous relaxed clock studies. Two clades identified as having transformed rate processes are the Vernonioid Clade and a clade within the Heliantheae alliance characterized by phytomelanic fruit (PF Clade) that represents an American radiation. In Africa, subfamilies Carduoideae, Pertyoideae, Gymnarrhenoideae, Cichorioideae, Corymbioideae, and Asteroideae diverged in a relatively short span of only 6.5millionyears during the Middle Eocene.
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            Resolution of deep nodes yields an improved backbone phylogeny and a new basal lineage to study early evolution of Asteraceae.

            A backbone phylogeny that fully resolves all subfamily and deeper nodes of Asteraceae was constructed using 14 chloroplast DNA loci. The recently named genus Famatinanthus was found to be sister to the Mutisioideae-Asteroideae clade that represents more than 99% of Asteraceae and was found to have the two chloroplast inversions present in all Asteraceae except the nine genera of Barnadesioideae. A monotypic subfamily Famatinanthoideae and tribe Famatinantheae are named herein as new. Relationships among the basal lineages of the family were resolved with strong support in the Bayesian analysis as (Barnadesioideae (Famatinanthoideae (Mutisioideae (Stifftioideae (Wunderlichioideae-Asteroideae))))). Ancestral state reconstruction of ten morphological characters at the root node of the Asteraceae showed that the ancestral sunflower would have had a woody habit, alternate leaves, solitary capitulescences, epaleate receptacles, smooth styles, smooth to microechinate pollen surface sculpturing, white to yellow corollas, and insect-mediated pollination. Herbaceous habit, echinate pollen surface, pubescent styles, and cymose capitulescences were reconstructed for backbone nodes of the phylogeny corresponding to clades that evolved shortly after Asteraceae dispersed out of South America. No support was found for discoid capitula, multiseriate involucres or bird pollination as the ancestral character condition for any node. Using this more resolved phylogenetic tree, the recently described Raiguenrayun cura+Mutisiapollis telleriae fossil should be associated to a more derived node than previously suggested when time calibrating phylogenies of Asteraceae.
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              Systematic Implications from Electron Microscopic Studies of Compositae Pollen-A Review

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                abb
                Acta Botanica Brasilica
                Acta Bot. Bras.
                Sociedade Botânica do Brasil (Belo Horizonte, BA, Brazil )
                0102-3306
                1677-941X
                January 2019
                : 33
                : 1
                : 128-134
                Affiliations
                [3] Brasília Distrito Federal orgnameEmbrapa Recursos Genéticos e Biotecnologia Brazil
                [4] Rio de Janeiro Rio de Janeiro orgnameUniversidade Federal do Rio de Janeiro orgdiv1Museu Nacional Brazil
                [2] Brasília Distrito Federal orgnameUniversidade de Brasília Brazil
                Article
                S0102-33062019000100128
                10.1590/0102-33062018abb0395
                ed02626d-9c1b-4264-933c-ab601a133310

                This work is licensed under a Creative Commons Attribution 4.0 International License.

                History
                : 10 November 2018
                : 18 December 2018
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 31, Pages: 7
                Product

                SciELO Brazil

                Categories
                Articles

                Verbesina,Brazil,Compositae,Heliantheae,morphology,pollen
                Verbesina, Brazil, Compositae, Heliantheae, morphology, pollen

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