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      The Efficient Extraction of Phenolic Compounds from Oak Gall Using a Miniaturized Matrix Solid-Phase Dispersion Method before their HPLC Determination

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          Abstract

          Background and Aim: Several gall varieties are found in Lorestan Province, Iran, on Quercus infectoria oak trees, which contain important phenolic compounds. In this work, a miniaturized matrix solid-phase dispersion (MSPD) extraction method has been developed for quantitative extraction and HPLC/UV determination of them. Materials and Methods: In the MSPD method, 10 mg of sample and 50 mg of silica gel adsorbent were transferred into an agate mortar. The mixture was finely pulverized after adding 40 µL dichloromethane as disperser solvent. It was then transferred into a cartridge, eluted by 350 µL of methanol, and the eluate was subsequently injected into HPLC for analysisn. Results: The extractions were quantitative with mean recoveries of 103.0±6.8% and 99.5±7.3% for ellagic acid (EA) and gallic acid (GA) in six replicated extractions, respectively. The detection limit of the method was 0.05-0.06 mg g-1. The method was successfully applied to the extraction and HPLC determination of the phenolic compounds in five gall species. Conclusion: The proposed technique is simple and fast. It substantially reduced the amounts of sample, sorbent and organic solvents required for the extraction. The maximum amounts of the phenolic compounds were found in Qalqaf and Bramazu galls.

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          Author and article information

          Journal
          Herbal Medicines Journal
          Lorestan University of Medical Sciences
          01 June 2017
          : 2
          : 2
          Article
          0f60626b97a74ad0b9e81430d8bab496
          10.22087/hmj.v0i0.615
          eda35a68-92b3-40e3-9af1-c735370dc3fb

          This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge. This work is licensed under a Creative Commons license (CC-BY). However, the license permits any user to read, copy, redistribute and make derivative the material in any medium or format for any purpose, even commercially. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/

          History
          Categories
          Pharmacy and materia medica
          RS1-441

          Medicine,Plant science & Botany,Analytical chemistry,Pharmacology & Pharmaceutical medicine,Life sciences,Infectious disease & Microbiology
          Phenolic compounds, Oak gall, Matrix solid-phase dispersion, Quercus infectoria, HPLC

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