Synergistic electrocatalytic activity of In2O3@FMWCNTs nanocomposite for electrochemical quantification of dobutamine in clinical patient blood and in injection dosage form

Abstract

Dobutamine (DBT) is a sympathomimetic amine drug that was designed as an inotropic agent for use in congestive heart failure. Hence, there was an impetus to develop a rapid and accurate method for monitoring the concentration of DBT within clinical samples. To address this critical need, a novel In2O3 and functionalized multi-walled carbon nanotubes nanocomposite (In2O3@FMWCNTs) was successfully prepared and applied in an electrochemical sensor to detect DBT. The resulting sensor displayed electrocatalytic toward the oxidation of DBT, which attributed to the synergistic effect of In2O3 and FMWCNTs. Electrochemical impedance spectroscopy (EIS) studies revealed that the smaller charge transfer resistance value (Rct) was observed at In2O3@FMWCNTs modified glassy carbon spherical (GCS) paste electrode (PE) as compared to that of In2O3NPs/GCSPE, FMWCNTs/GCSPE and GCSPE, which authenticates its good conductivity. Furthermore, the calculated value of standard rate constant (ks) for the modified electrode demonstrates the fast electron transfer between DBT and the electrode surface. The fabricated electrochemical sensor indicated high selectivity and sensitivity for DBT determination over the oxidation of uric acid and ascorbic acid. The limit of detection of DBT at In2O3@FMWCNTs/GCSPE was found to be 1.42 × 10-10 M. The proposed sensor is effectively used for the detection of DBT in biological fluids, clinical patient blood and in injection dosage form.