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      First report and molecular characterization of the dagger nematode, Xiphinema oxycaudatum (Nematoda, Dorylaimidae) from South Africa

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          Abstract

          Abstract

          Plant-parasitic nematodes of the genus Xiphinema Cobb, 1913 comprise a complex group of nematode species, some of which are important vectors of plant viruses. During a field survey to determine the soil health of an abandoned honeybush ( Cyclopia genistoides ) monoculture, a high density of the dagger nematode, Xiphinema oxycaudatum Lamberti & Bleve-Zacheo, 1979 ( Nematoda , Dorylaimidae ), was observed in soil around the roots of honeybush plants in an abandoned farmland at Bereaville, an old mission station in the Western Cape province of South Africa. Soil samples were taken from the rhizosphere of plants and nematodes were extracted from the soil using a modified extraction tray method. Specimen of the dagger nematodes were processed for scanning electron microscopy, morphological and molecular analysis. Molecular profiling of the nematode species was done in order to give an accurate diagnosis and to effectively discriminate the nematode from other species within the Xiphinema americanum group. Phylogenetic analysis based on the D2D3 expansion segment of the 28S gene supported a close relationship of species within the americanum group, however, the protein-coding cytochrome oxidase ( coxI) of the mitochondrial gene provided a useful tool for distinguishing the nematode from other species within the group. This study represents the first report of X. oxycaudatum from South Africa.

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          Most cited references 24

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          Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

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            A molecular phylogenetic approach to Longidoridae (Nematoda: Dorylaimida)

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              Phylogeny, diversity, and species delimitation in some species of the Xiphinema americanum-group complex (Nematoda: Longidoridae), as inferred from nuclear and mitochondrial DNA sequences and morphology

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                Author and article information

                Contributors
                Journal
                Zookeys
                Zookeys
                2
                urn:lsid:arphahub.com:pub:45048D35-BB1D-5CE8-9668-537E44BD4C7E
                urn:lsid:zoobank.org:pub:91BD42D4-90F1-4B45-9350-EEF175B1727A
                ZooKeys
                Pensoft Publishers
                1313-2989
                1313-2970
                2019
                03 December 2019
                : 894
                : 1-17
                Affiliations
                [1 ] Department of Conservation Ecology and Entomology, Private Bag X1, Matieland 7602, Stellenbosch, South Africa Department of Conservation Ecology and Entomology Stellenbosch South Africa
                [2 ] Plant Protection Division, Agriculture Research Council (ARC) Infruitec-Nietvoorbij, Private Bag X5026, Stellenbosch 7599, South Africa Plant Protection Division, Agriculture Research Council Stellenbosch South Africa
                [3 ] Nematology Unit, Biosystematics Division, ARC-Plant Protection Research Institute, Private Bag X134, Queenswood 0121, South Africa ARC-Plant Protection Research Institute Queenswood South Africa
                [4 ] Department of Zoology, University of Johannesburg, PO Box 524, Auckland Park, Johannesburg 2006, South Africa University of Johannesburg Johannesburg South Africa
                Author notes
                Corresponding author: Fisayo Y. Daramola ( fdaramola@ 123456sun.ac.za )

                Academic editor: Sergei Subbotin

                Article
                35281
                10.3897/zookeys.894.35281
                6904367
                Fisayo Y. Daramola, Rinus Knoetze, Antoinette Swart, Antoinette P. Malan

                This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

                Funding
                The Human resources for Industry Programme
                Categories
                Short Communication
                Dorylaimidae
                Nemata
                Agriculture and Forestry
                Biodiversity & Conservation
                Cenozoic
                Africa

                Animal science & Zoology

                molecular identification, honeybush, d2d3, coxi

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