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Abstract
Multi-functional cellulase gene mfc was expressed in Coprinus cinereus under naturally
non-inductive conditions using three heterologous promoters. Endo-beta-1,4-glucanase
expression was achieved in solid and liquid media with promoter sequences from the
Lentinula edodesgpd gene, the Flammulina velutipes gpd gene and the Volvariella volvaceagpd
gene. As measured by enzyme activity in liquid cultures, a 613-bp gpd promoter fragment
from L. edodes was most efficient, followed by a 752-bp gpd fragment from F. velutipes.
The V. volvacea gpd promoter sequence was less active, in comparison. Irrespective
of the promoter used, enzymatic activities increase 34-fold for highly active transformants
and 29-fold for less active one by using cellulase-inducing medium. The highest activities
of endo-beta-1,4-glucanase (34.234 U/ml) and endo-beta-1,4-xylanase (263.695 U/ml)
were reached by using the L. edodesgpd promoter.