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      Immunoregulatory Effects of Glandular Kallikrein from the Salivary Submandibular Gland of Rats

      , ,

      Neuroimmunomodulation

      S. Karger AG

      Delayed hypersensitivity, Salivary glands, Kallikrein

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          Abstract

          A protein of 40 kD molecular weight was isolated from the salivary submandibular glands of male rats. The protein catalyzed the hydrolysis of α-N-benzoyl- L-arginine ethyl ester. This esterase activity was inhibitable with the protease inhibitor aprotinin. The sequence of the first 25 amino acids of this protein was identical to that of rat glandular kallikrein (rGK). When added to cultures of murine lymph node cells suboptimally stimulated with the T cell mitogen concanavalin A, rGK markedly stimulated the proliferative activity of these cells. When injected into mice, rGK suppressed the contact sensitivity response to picryl chloride, a form of delayed-type hypersensitivity. Similar in vitro and in vivo effects were induced with GK from porcine pancreas (pGK). Moreover, the aforementioned in vitro and in vivo effects were abolished by aprotinin either added to the tissue culture medium or injected into the animals immediately before rGK or pGK. This demonstrates that the enzymatic activity of rGK and pGK is important for the induction of immunoregulatory effects. These results suggest that rGK is a systemic immunoregulatory enzyme with immunosuppressive potential. GK is the first example for systemic immunoregulation by an enzyme, the secretion of which is under neuroendocrine control.

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          Author and article information

          Journal
          NIM
          Neuroimmunomodulation
          10.1159/issn.1021-7401
          Neuroimmunomodulation
          S. Karger AG
          1021-7401
          1423-0216
          1997
          1997
          03 December 1997
          : 4
          : 2
          : 107-112
          Affiliations
          Department of Immunology, University of Manitoba, Winnipeg, Canada
          Article
          97328 Neuroimmunomodulation 1997;4:107–112
          10.1159/000097328
          9483202
          © 1997 S. Karger AG, Basel

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          Page count
          Pages: 6
          Categories
          Original Paper

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